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Biodegradation in water: screening tests

In order to assess the biotic degradation, a ready biodegradability test was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test according to slightly modified OECD and EEC Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. In the biodegradability tests, microorganisms are inoculated into a chemically defined liquid medium containing the test substance as sole carbon and energy source. The test compound is exposed to the inoculum, consisting of relatively low numbers of microorganisms from an activated sludge plant, under aerobic conditions for a period of at least 28 days.The test was prolonged because the pass level was not reached at day 28. The test substance caused no reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum. The biodegradation of the test substance was 4%. Hence, the test substance was not biodegraded in the Closed Bottle test (28 days and prolonged) and should therefore no be classified as readily biodegradable. The reference substance, sodium acetate, was degraded 69% of its theoretical oxygen demand after 14 days.