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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The authors tested acute toxicity by inhalation according to a methodology described by Smyth H.F. and Carpenter C.P. GLP standards are not specified and few details are given on material and methods. However, results are clearly described and as a result this study is considered reliable with restrictions because it meets generally accepted scientific principles and is acceptable for assessment (Klimisch 2e)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Range-finding toxicity data: list IV
Author:
Smyth H.F., Carpenter C.P. and Weil C.S.
Year:
1951
Bibliographic source:
Archives of Industrial Hygiene and Occupational Medicine, 4: 119-122
Reference Type:
publication
Title:
Further experience with the range finding test in the industrial toxicology laboratory
Author:
Smyth H. F. and Carpenter C. P.
Year:
1948
Bibliographic source:
Journal of industrial hygiene and toxicology, 30, 63-68.

Materials and methods

Principles of method if other than guideline:
Methodology according to Smyth H.F. and Carpenter C.P. published in Journal of Industrial Hygiene and Toxicology (1948) 30: 63-68
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): benzotrichloride (alpha, alpha, alpha-trichlorotoluene)

No more data available

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
other: no data
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
125 ppm ( about 1 mg/L)
No. of animals per sex per dose:
6
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
other: mortality
Effect level:
125 ppm
Exp. duration:
4 h
Remarks on result:
other: 83.3% mortality in 14 days
Sex:
not specified
Dose descriptor:
LC50
Effect level:
< 125 ppm
Exp. duration:
4 h
Sex:
not specified
Dose descriptor:
other: mortality
Effect level:
125 ppm
Exp. duration:
30 min
Remarks on result:
other: maximum duration of exposure to saturated vapour at which no deaths occured

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: not specified
Conclusions:
The authors tested acute toxicity by inhalation of benzotrichloride according to a methodology described by Smyth H.F. and Carpenter C.P. Under the test conditions, 83.3% deaths were recorded in 14 days after an exposure of 4h to 125 ppm. Hence the LC50 (4h) is lower than 125 ppm, which is about 1 mg/L. Thus, according to the CLP regulation (EC) N° 1272/2008 benzotrichloride could be considered at least a category 3 (toxic if inhaled) as the LC50 (4h) is below 1 mg/L and since the the authors created a mist of the test substance.
Executive summary:

The authors tested acute toxicity by inhalation of benzotrichloride (CAS n° 98-07-7) according to a methodology described by Smyth H.F. and Carpenter C.P. [Journal of Industrial Hygiene and Toxicology (1948) 30: 63-68]. The authors created a "saturated vapour" by creating a mist by bubbling air and exposed the rats during four hours to this mist.

Two experiments consisted in checking: (1) the fractional mortality in 14 days as a result of a 4h exposure to 125 ppm of the test substance using 6 rats and (2) the maximum exposure duration to saturated vapour at which no deaths could be recorded. No analytical monitoring was performed over the period of exposure. The exposure concentration was estimated by the flow of air through the test substance.

Under the test conditions, 83.3% deaths were recorded in 14 days after an exposure of 4h to 125 ppm. Hence the LC50 (4h) is lower than 125 ppm, which is about 1 mg/L. Furthermore the maximum exposure duration to "saturated vapour" at which no deaths were recorded was 30 minutes. Thus, according to the CLP regulation (EC) N° 1272/2008 benzotrichloride could be considered at least a category 3 (toxic if inhaled) as the LC50 (4h) is below 1 mg/L and since the the authors created a mist of the test substance.

GLP standards are not specified and few details are given on material and methods. However, results are clearly described and as a result this study is considered reliable with restrictions because it meets generally accepted scientific principles and, thus, is acceptable for assessment (Klimisch 2e)