α,α,α-trichlorotoluene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The authors tested acute toxicity by inhalation according to a methodology described by Smyth H.F. and Carpenter C.P. GLP standards are not specified and few details are given on material and methods. However, results are clearly described and as a result this study is considered reliable with restrictions because it meets generally accepted scientific principles and is acceptable for assessment (Klimisch 2e)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-finding toxicity data: list IV

Author:

Smyth H.F., Carpenter C.P. and Weil C.S.

Year:

1951

Bibliographic source:

Archives of Industrial Hygiene and Occupational Medicine, 4: 119-122

Reference 2

Reference Type:

publication

Title:

Further experience with the range finding test in the industrial toxicology laboratory

Author:

Smyth H. F. and Carpenter C. P.

Year:

1948

Bibliographic source:

Journal of industrial hygiene and toxicology, 30, 63-68.

Materials and methods

Principles of method if other than guideline:

Methodology according to Smyth H.F. and Carpenter C.P. published in Journal of Industrial Hygiene and Toxicology (1948) 30: 63-68

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trichlorotoluene

EC Number:

202-634-5

EC Name:

α,α,α-trichlorotoluene

Cas Number:

98-07-7

Molecular formula:

C7H5Cl3

IUPAC Name:

α,α,α-trichlorotoluene

Constituent 2

Reference substance name:

trichloromethylbenzene

IUPAC Name:

trichloromethylbenzene

Details on test material:

- Name of test material (as cited in study report): benzotrichloride (alpha, alpha, alpha-trichlorotoluene)

No more data available

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: no data

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

125 ppm ( about 1 mg/L)

No. of animals per sex per dose:

6

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

other: mortality

Effect level:

125 ppm

Exp. duration:

4 h

Remarks on result:

other: 83.3% mortality in 14 days

Effect levels 2

Sex:

not specified

Dose descriptor:

LC50

Effect level:

< 125 ppm

Exp. duration:

4 h

Effect levels 3

Sex:

not specified

Dose descriptor:

other: mortality

Effect level:

125 ppm

Exp. duration:

30 min

Remarks on result:

other: maximum duration of exposure to saturated vapour at which no deaths occured

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: not specified

Conclusions:

The authors tested acute toxicity by inhalation of benzotrichloride according to a methodology described by Smyth H.F. and Carpenter C.P. Under the test conditions, 83.3% deaths were recorded in 14 days after an exposure of 4h to 125 ppm. Hence the LC50 (4h) is lower than 125 ppm, which is about 1 mg/L. Thus, according to the CLP regulation (EC) N° 1272/2008 benzotrichloride could be considered at least a category 3 (toxic if inhaled) as the LC50 (4h) is below 1 mg/L and since the the authors created a mist of the test substance.

Executive summary:

The authors tested acute toxicity by inhalation of benzotrichloride (CAS n° 98-07-7) according to a methodology described by Smyth H.F. and Carpenter C.P. [Journal of Industrial Hygiene and Toxicology (1948) 30: 63-68]. The authors created a "saturated vapour" by creating a mist by bubbling air and exposed the rats during four hours to this mist.

Two experiments consisted in checking: (1) the fractional mortality in 14 days as a result of a 4h exposure to 125 ppm of the test substance using 6 rats and (2) the maximum exposure duration to saturated vapour at which no deaths could be recorded. No analytical monitoring was performed over the period of exposure. The exposure concentration was estimated by the flow of air through the test substance.

Under the test conditions, 83.3% deaths were recorded in 14 days after an exposure of 4h to 125 ppm. Hence the LC50 (4h) is lower than 125 ppm, which is about 1 mg/L. Furthermore the maximum exposure duration to "saturated vapour" at which no deaths were recorded was 30 minutes. Thus, according to the CLP regulation (EC) N° 1272/2008 benzotrichloride could be considered at least a category 3 (toxic if inhaled) as the LC50 (4h) is below 1 mg/L and since the the authors created a mist of the test substance.

GLP standards are not specified and few details are given on material and methods. However, results are clearly described and as a result this study is considered reliable with restrictions because it meets generally accepted scientific principles and, thus, is acceptable for assessment (Klimisch 2e)