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Description of key information

- Acute oral toxicity: LD 50 = 1590 mg / kg bw  for female and 2188 mg / kg bw  for male 

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Dose descriptor:
LD50
1 590 mg/kg bw

Additional information

The discussion is focussed on 'Acute oral toxicity' only as this is a required endpoint for 'Transported isolated intermediates under strictly controlled conditions above 1000 tpa.

Acute oral toxicity:

Two studies are available for this endpoint. One is based on the standard acute testing method and very similar to the OECD guidleine 401 and the other older is based on a methodology similar.

In the study report from Mihail (1978), the acute oral toxicity of benzotrichloride (CAS n° 98 -07 -7) was investigated using male and female Wistar rats (n=15 per sex and dose group) in a test very similar to the OECD guideline 401. The authors treated the rats by gavage with the test substance formulated in water containing Cremophor EL. Mortality and clinical signs were investigated during fourteen days after the exposure. All deceased animals were checked for gross pathology symptoms of intoxication. LD 50 were estimated by probit analysis.

LD50 values of 2,188 and 1,590 mg/kg bw were found for male and female rats, respectively. During the observation period of fourteen days, reduced activity, scrubby fur, labored breathing, and polyuria with sanguineous urine were observed as toxicological symptoms. Symptoms started within 15 minutes after treatment and persisted for 7 to 9 days. The gross pathology of deceased animals revealed empty intestinal tracts and white stipples on the stomach mucosa.

In the other available study from Smyth (1951), the authors tested oral toxicity of benzotrichloride (CAS n° 98-07-7) according to a methodology described by Smyth H.F. and Carpenter C.P. [Journal of Industrial Hygiene and Toxicology (1948) 30: 63-68]. Rats were orally exposed via an unspecified route with a single dose (concentration also unknown). In these test conditions, the LD50 after 14 days was 6000 mg/kg. No further examinations of the tested rats are reported. Very few data was reported on this experiment, and thus this study is considered as not assignable.

Based on these results, the choice of the key study was clear since only one relevant and reliable data was available.

Justification for classification or non-classification

According to the criteria of the CLP regulation n° 1272/2008, the benzotrichloride should be considered as an acute toxic category 4 since the LD 50 for rats following a single oral administration is between 300 and 2000 mg / kg bw.