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EC number: 217-614-1 | CAS number: 1908-87-8
Results after treatment with 3-Methyl-thiazolidin-2-thion and the controls:
Absor-bance 570 nm Tissue 1*
Absor-bance 570 nm Tissue 2*
Absor-bance 570 nm Tissue 3*
Mean Absor-bance of 3 Tissues
Rel. Absor-bance [%] Tissue 1, 2 + 3**
Relative Standard Deviation
Mean Rel. Absorbance
[% of Negative Control]***
* Mean of three replicate wells after blank correction
** relative absorbance per tissue [rounded values]: 100 x (absorbancetissue) / (mean absorbancenegative control)
*** relative absorbance per treatment group [rounded values]: 100 x (mean absorbancetest item / negative control) / (mean absorbancenegative control)
The optical pre-experiment (colour interference pre-experiment) to investigate the test item’s colour change potential in water did not led to a change in colour.
Optical evaluation of the MTT-reducing capacity of the test item after 1 hour incubation with MTT-reagent did not show blue colour.
The mean relative absorbance value of the test item, corresponding to the cell viability, decreased to 88.9% (threshold for irritancy: ≤ 50%), consequently the test item was not irritant to skin.
This in vitro study was performed to assess the irritation potential of 3-Methyl-thiazolidin-2-thion by means of the Human Skin Model Test.
The test item did not reduce MTT (test for direct MTT reduction), and it did not change colour when mixed with deionised water (test for colour interference). Also its intrinsic colour was not intensive. Consequently, additional tests with freeze-killed or viable tissues were not necessary.
Each three tissues of the human skin model EpiDerm™ were treated with the test item, the negative or the positive control for 60 minutes.
Approximately 25 mg of the test item were applied to each tissue, wetted with 50 μL of DPBS, and spread to match the surface of the tissue.
30 μL of either the negative control (DPBS) or the positive control (5% SLS) were applied to each tissue.
After treatment with the negative control the absorbance values were well in the required range of the acceptability criterion of mean OD ≥ 0.8 and ≤ 2.8 for the 60 minutes treatment interval, thus assuring the quality of the tissues.
Treatment with the positive control induced a sufficient decrease in the relative absorbance as compared to the negative control for the 60 minutes treatment interval, and thus assuring the validity of the test system.
After treatment with the test item 3-Methyl-thiazolidin-2-thion the mean relative absorbance value decreased irrelevantly to 88.9% compared to the relative absorbance value of the negative control. This value is above the threshold for irritancy of ≤ 50%. Therefore, the test item is not considered to possess an irritant potential.
In conclusion, it can be stated that in this study and under the experimental conditions reported, 3-Methyl-thiazolidin-2-thion is not irritant to skin.
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