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EC number: 226-375-2 | CAS number: 5382-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial specific surface area
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- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer reviewed journals
Data source
Reference
- Reference Type:
- publication
- Title:
- Chemical burns of the rabbit cornea.
- Author:
- Carpenter, C.P., Smyth, H.F.
- Year:
- 1 946
- Bibliographic source:
- American Journal of Ophthalmology 29, 1363–1372, 1946
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- To assess the ocular irritation potential of 2-(4-Morpholinyl)ethanol
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-morpholinoethanol
- EC Number:
- 210-734-5
- EC Name:
- 2-morpholinoethanol
- Cas Number:
- 622-40-2
- Molecular formula:
- C6H13NO2
- IUPAC Name:
- 2-(4-Morpholinyl)ethanol
- Test material form:
- liquid
- Details on test material:
- Name of the test chemical: 2-(4-Morpholinyl)ethanol
Common Name: N-(2-Hydroxyethyl)morpholine
Molecular Weight: 131.174 g/mol
Molecular Formula: C6H13NO2
InChI: 1S/C6H13NO2/c8-4-1-7-2-5-9-6-3-7/h8H,1-6H2
Substance Type: Organic
Physical State: Liquid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: 2-(4-Morpholinyl)ethanol
Common Name: N-(2-Hydroxyethyl)morpholine
Molecular Weight: 131.174 g/mol
Molecular Formula: C6H13NO2
InChI: 1S/C6H13NO2/c8-4-1-7-2-5-9-6-3-7/h8H,1-6H2
Substance Type: Organic
Physical State: Liquid
Test animals / tissue source
- Species:
- rabbit
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- no data available
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- 0.005 ml. of the undiluted material
- Duration of treatment / exposure:
- 0.005 ml. of the undiluted material is applied to the center of the cornea while the lids are retracted.
- Observation period (in vivo):
- Eighteen to 24 hours
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- no data available
- Details on study design:
- SCORING SYSTEM: 24 hours after the application of the test chemical
Results and discussion
In vitro
- Other effects / acceptance of results:
- no data available
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 5
- Max. score:
- 10
- Reversibility:
- not specified
- Remarks on result:
- probability of severe irritation
- Irritant / corrosive response data:
- Severely irritating to rabbit eyes.
Any other information on results incl. tables
Table 1: System for numerical scoring of injury to the rabbit eye 24 hours after application of material
Symptoms |
Points |
Maximum score |
Symptom Visible Before Fluorescein Staining |
|
|
Cornea dull |
2 |
|
Cornea opaque, less than half of area |
4 |
|
Cornea opaque, more than half of area |
6 |
6 |
Keratoconus |
|
6 |
Iritis, slight internal congestion |
1 |
|
Iritis, marked internal congestion |
2 |
2 |
Symptoms visible after fluorescein staining |
|
|
Necrosis on less than 5% of cornea |
1 |
|
Necrosis on 5 to12% |
2 |
|
Necrosis on 12 to 37% |
3 |
|
Necrosis on 38 to 62% |
4 |
|
Necrosis on 63 to 87% |
5 |
|
Necrosis on 88 to 100% |
6 |
6 |
Total |
|
20 |
Table 2: Grades of injury employed for rating the relative damage produced by chemicals in the eye
Injury grade |
Definition |
1 |
0.5 ml undiluted gives injury of 0 to 10 points |
2 |
0.5 ml undiluted gives injury of over 1 up to 5 points |
3 |
0.1 ml undiluted gives injury of up to 5 points (0.5 ml gives over 5) |
4 |
0.02 ml undiluted gives injury of up to 5 points (0.1ml gives over 5) |
5 |
0.005 ml undiluted gives injury of up to 5 (0.02 ml gives over 5) |
6 |
Excess of 40% solution gives injury up to 5 (0.005 ml gives over5) |
7 |
Excess of 15% solution gives injury of up to 5 points (40% gives over 5.0) |
8 |
Excess of 5% solution gives injury of up to 5 points (15% gives over 5) |
9 |
Excess of 1% solution gives injury up to 5 points (5% gives over 5) |
10 |
Excess of 1% solution gives injury up to 5 points |
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- A rabbit eye irritation test was conducted to assess the irritation potency of 2-(4-Morpholinyl)ethanol. 2-(4-Morpholinyl)ethanol was grouped under Grade 5 injury (0.005 ml undiluted gives injury of up to 5 (0.02 ml gives over 5)) when rabbit eyes were observed for injuries. Based on the grades, it can be inferred that 2-(4-Morpholinyl)ethanol was severely irritating to rabbit eyes.
- Executive summary:
A rabbit eye irritation test was conducted to assess the irritation potency of 2-(4-Morpholinyl)ethanol.
Normal albino rabbit eyes were selected on the basis of absence of grossly visible staining by a 5-percent aqueous solution of fluorescein sodium, flushed with distilled water 20 seconds after application. A 2 hour interval was given so that the eye could return back to normal condition. Then, 0.005 ml of the undiluted test material was applied to the center of the cornea while the lids were retracted. About one minute later, the lids were released. This procedure is necessary to prevent the removal of a portion of the dose by the very efficient wiping system of the lids before intimate contact has been made with the eye. 18 to 24 hours later, the eyes were examined in strong diffuse daylight, then stained with fluorescein, and the injury was scored.
2-(4-Morpholinyl)ethanol was grouped under Grade 5 injury (0.005 ml undiluted gives injury of up to 5 (0.02 ml gives over 5)) when rabbit eyes were observed for injuries. Based on the grades, it can be inferred that 2-(4-Morpholinyl)ethanolwas severely irritating to rabbit eyes.
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