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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from peer reviewed journals

Data source

Reference
Reference Type:
publication
Title:
Range-Finding Toxicity Data: List VI
Author:
Henry F. Smyth Jr., Charles P. Carpenter, Carrol S. Well, Urbano C. Pozzani & Jean A. Striegel
Year:
1962
Bibliographic source:
American Industrial Hygiene Association Journal, 1962, 23:2, 95-107

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Primary dermal irritation study was carried out on rabbits to assess the irritation potential of 1-methylpiperazine
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Name of test material (as cited in study report): 1-methyl piperazine
Molecular formula : C5H12N2
Molecular weight : 100.1638 g/mol
Substance Type: Organic
Physical State: Liquid
Specific details on test material used for the study:
Name of test material (as cited in study report): 1-methyl piperazine
Molecular formula : C5H12N2
Molecular weight : 100.1638 g/mol
Substance Type: Organic
Physical State: Liquid

Test animals

Species:
rabbit
Strain:
other: albino
Details on test animals and environmental conditions:
no data available

Test system

Type of coverage:
open
Preparation of test site:
clipped
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
0.01 ml of undiluted sample or of solutions in water, acetone, propylene glycol
Duration of treatment / exposure:
24 hours
Observation period:
24 hours
Number of animals:
5
Details on study design:
TEST SITE
Area of exposure: closely clipped, intact abdominal skin
% coverage: No data
Type of wrap if used: no wrap used

REMOVAL OF TEST SUBSTANCE
Washing (if done): no data
Time after start of exposure: no data

SCORING SYSTEM:
Primary skin irritation on rabbits was recorded in a 10-grade ordinal series. Grade 1 indicates no irritation and Grade 2 the least visible capillary injection from the undiluted chemical. Grade 6 indicates necrosis when undiluted and Grade 10 indicates necrosis from a 0.01% solution

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
24 h
Score:
6
Max. score:
10
Reversibility:
not specified
Remarks on result:
probability of moderate irritation
Irritant / corrosive response data:
Primary Irritation score after 24 hours for 1-methylpiperazine was Grade 6

Any other information on results incl. tables

 Table: Results of the irritation study

Material tested

Irritation on uncovered rabbit belly

1 -methylpiperazine

6

                                                    

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritant) based on GHS criteria
Conclusions:
Acute dermal irritation study was carried out to assess the irritation potential of 1-methyl piperazine.
Primary Irritation score after 24 hours for 1-methylpiperazine was Grade 6.
Based on this grade, 1-methyl piperazine can be considered moderately irritating to rabbit skin.
Executive summary:

Acute dermal irritation study was carried out to assess the irritation potential of 1-methylpiperazine.

Primary skin irritation on rabbits was recorded in a 10-grade ordinal series and was based upon the severest reaction that develops on the clipped abdominal skin of each of five albino rabbits within 24 hours of the uncovered application of 0.01 ml of undiluted sample or of solutions in water, propylene glycol, or acetone.

Grade 1 indicates no irritation and Grade 2 the least visible capillary injection from the undiluted chemical. Grade 6 indicates necrosis when undiluted and Grade 10 indicates necrosis from a 0.01% solution

Primary Irritation score after 24 hours for 1 -methyl piperazine was Grade 6.

Based on this grade,1-methylpiperazine can be considered moderately irritating to rabbit skin.