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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1992
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Basic data given, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
None
GLP compliance:
no
Analytical monitoring:
not specified
Details on sampling:
None
Vehicle:
no
Details on test solutions:
Not available
Test organisms (species):
Daphnia magna
Details on test organisms:
Not available
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not available
Hardness:
Not available
Test temperature:
Not available
pH:
Not available
Dissolved oxygen:
Not available
Salinity:
Not available
Nominal and measured concentrations:
0, 0.01, 0.1, 1.0, 10 and 100 mg/L
Details on test conditions:
Not available
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Not available
Results with reference substance (positive control):
Not available
Reported statistics and error estimates:
Not available

None

Validity criteria fulfilled:
no
Conclusions:
EC50 in Daphnia magna at 48 h : >100 mg/L
Executive summary:

EC50 in Daphnia magna at 48 h : >100 mg/L

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1992
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Basic data given, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
None
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
None
Analytical monitoring:
not specified
Details on sampling:
None
Vehicle:
no
Details on test solutions:
Not available
Test organisms (species):
Daphnia magna
Details on test organisms:
Not available
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not available
Hardness:
Not available
Test temperature:
Not available
pH:
Not available
Dissolved oxygen:
Not available
Salinity:
Not available
Nominal and measured concentrations:
Not available
Details on test conditions:
Not available
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Not available
Results with reference substance (positive control):
Not available
Reported statistics and error estimates:
Not available

None

Validity criteria fulfilled:
no
Conclusions:
EC50 in Daphnia magna at 48 h : >100 mg/L
Executive summary:

EC50 in Daphnia magna at 48 h : >100 mg/L

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given, acceptable for assessment
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that the source and the target substance have very similar physicochemical and (eco)toxicological properties because their chemical structures are nearly identical. An analogue approach has thus been employed. The target substance is the meta-isomer of the dye Reactive Blue 049, where the sulphonate group is bound at the meta-position of the aminobenzene moiety. The source chemical is the reaction mass of both the meta-isomer and the para-isomer of Reactive Blue 049.
The presence of sulphonate groups make both dyes highly water soluble and therefore less critical for human health and environmental issues. Based on their chemical similarity, similar properties are expected in both humans and the environment.

2. SOURCE AND TARGET CHEMICAL(S)
Source: Reactive Blue 49 meta/para (CAS# 72214-18-7 / EC# 276-481-8)
Target: Reactive Blue 49 meta (CAS# 72927-99-2 / EC# 277-040-2)

3. ANALOGUE APPROACH JUSTIFICATION
see attachment under 4.2 Melting point / freezing point
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Conclusions:
EC50 in Daphnia magna at 48 h : >100 mg/L (2 studies)
Executive summary:

Two weight of evidence studies showed that the source substance does not have acute toxic effects on Daphnia magna up to 100 mg/L which leads to a 48h-EC50 greater than 100 mg/L. The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 48h-EC50 will be >100 mg/L as well.

Description of key information

The test item had no acute toxicity effects on Daphnia magna with an 48-hour EC50 > 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Two weight of evidence studies showed that the source substance does not have acute toxic effects on Daphnia magna up to 100 mg/L which leads to a 48h-EC50 greater than 100 mg/L. The structurally related target substance will show the same behaviour and therefore it can be anticipated that the 48h-EC50 will be >100 mg/L as well.