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Environmental fate & pathways

Adsorption / desorption

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Description of key information

The test substance does not adsorb to sludge or soil

Key value for chemical safety assessment

Koc at 20 °C:

Additional information

The adsorption coefficient of Reactive Black 5 was determined according to the “HPLC- method” described in the European Commission Regulation (EC) No. 440/2008. 

The adsorption coefficient of the test item was found to be:                      log KOC    < 1.25


A study was conducted to determine the adsorption coefficient of the Structural Analogue 01 according to the "HPLC method" described in EU Method C.19 and OECD Guideline 121, in compliance with GLP.

The test substance was injected twice into an HPLC column where the stationary phase was composed of LiChrospher 100 CN (5 µm), and methanol/buffer pH 8.0 (30:70 v/v) was used as the mobile phase. A calibration graph was drawn by measuring the retention times of a series of substances with known adsorption coefficients. The adsorption coefficient of the test substance was derived by comparing its retention time with the calibration curve. Detection of the test substance was carried out at 598 nm.

Under the conditions of the study, the adsorption coefficient (log Koc) of the test substance was determined to be 0.60. 

[LogKoc: 0.6]