Slags, steelmaking

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004-2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

2007

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Slags, steelmaking

EC Number:

266-004-1

EC Name:

Slags, steelmaking

Cas Number:

65996-71-6

Molecular formula:

~ Al(n)Ca(m)Fe(o)Mg(p)Si(q)O(3n/2+m+o+p+2q)

IUPAC Name:

Aluminium-Calcium- Iron-Magnesium-Silicium oxide equivalent

Details on test material:

In order to substantiate this evaluation, the Tecarn-Tecnologia Ambiental Ltda accredited before the Ministry of Agriculture and is holder of bio-safety certificates.
SMS samples were collected in CST´s processing plants. The crushed stone is from Brasitalia quarry, located in the municipality of Serra, Brasilia. Crushed stone samples were collected and prepared by the company Kaerne Engenharia Ltda. In compliance with the method defined in NBR 10.007.2007.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

water

Duration of exposure:

24 h, consecutive observation period 14 d

Doses:

their skin treated with the maximum dosage recommended for each product (4000 mg/kg)

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

The animals were individually observed with special attention during the first 24 h after the administration of the test substance and for a period of 14 d when they were observed 7 d a week if evident signs were found, or 5 d a week when toxicity signs were not evident. The symptoms to be observed were alterations in the skin, fur, eyes and mucous membranes, dyspnoea, behaviour alterations, tremors, convulsions, salivation, diarrhea, lethargy, sleepiness, coma and death. In the case of mortality, the records include the approximate time of death.

Results and discussion

Preliminary study:

As it was known that there is no evidence of dermal toxicity, main test was made with maximum possible concentration of slags and crushed natural stone

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 4 000 mg/kg bw

Based on:

test mat.

Mortality:

no mortality reported

Clinical signs:

other: no clinical signs reported

Gross pathology:

no data

Other findings:

The monitoring of the animals included observation for alternations in the skin, fur, eyes and mucous membranes dyspnoea, behaviour alternations, tremors, convulsions, salivation, diarrhoea, lethargy, sleepiness, coma, and death. In case of death, the time of death would have been reported.

Any other information on results incl. tables

SMS LD₅₀ > 4000 mg/kg

Crushed stone: LD₅₀ > 4000 mg/kg

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

SMS: rats OECD 402: LD50 > 4000 mg/kg
Crushed natural stone: rats OECD 402: LD50 > 4000 mg/kg

Executive summary:

To test the dermal toxicity of slags, steelmaking (SMS), and crushed stone from a quarry in the vicinity of Serra, Brasilia, tests were performed following OECD guideline 402 in Wistar rats. Fine-ground SMS and crushed stone were dosed, in their original form, at 4 g/kg bw (body weight) under semi-occlusive dressing for 24 h. After dosing, the patches were removed and the animals were observed for a post exposure period of 14 days. The LD₅₀ was > 4000 mg/kg bw for each test material.

Slags, steelmaking (SMS), and natural stone from a quarry in Serra, Brasilia, are not toxic via the dermal route, and do not need to be classified as dermal toxicants. No signal word and no hazard statement is required.