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Toxicity to microorganisms

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Description of key information

ABS: Vibrio fischeri Microtox: 30 min-EC50 > 80 g/L

Key value for chemical safety assessment

EC50 for microorganisms:
80 g/L
EC10 or NOEC for microorganisms:
80 g/L

Additional information

In two acute toxicity tests according to the Microtox procedure, undiluted, neutralized leachate of ABS (DIN 38414 -4/DEV-S4, L/S 10/1) had no effect on the bioluminescence of the marine proteobacterium, Vibrio fischeri. After 30 min of incubation there was no decrease in biofluorescence, and the EC50 is larger than 80 g/L (nominal), the highest concentration tested (BfG 2008).

In an acute toxicity test according to the Microtox procedure, undiluted, neutralized leachate of ABS (L/S 10/1) had no effect on the bioluminescence of the marine proteobacterium, Vibrio fischeri. After 30 min of incubation, there was no decrease in biofluorescence, and the NOEC was >= 90 g/L, and the EC50 was larger than 90 g/L (nominal), the highest concentration tested (LECES 1999).

Leachate of air-cooled blast furnace slag (ABS, subtype toploader kiln slag) is not inhibitory on the bioluminecence of the marine bacterium, Vibrio fischeri, in a test according to ISO 11348. The 30 min-IC0 is >= 100 mg/L (nominal, highest concentration tested), the IC50 is > 100 mg/L (Hygiene-Institut des Ruhrgebiets 2008).

This value is disregarded for assessment because the insolubility of the slag is a desired property of the slag for its use in construction. The low solubility was the reason why the experiments were ordered to be made with extrem low concentrations instead of concentrations relevant for the environment.