Methyl methacrylate

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

static inhalation exposure with unclear exposure; extremely high exposure; partially no dose relationship; Chinese publication with English summary

Data source

Reference

Reference Type:

publication

Title:

Toxic effect of MMA to the reproductive system of male mice

Author:

Wang C., Chen X, Cao S.

Year:

2011

Bibliographic source:

Zhiye Yu Jiankang (2011), 27(9), 970-972

Materials and methods

Principles of method if other than guideline:

To study the toxic effect of methylmethacrylate (MMA) to reproductive system of the male mammals.Through sperm shape abnormality test of the rats mice and the examinations of the testicular and seminal vesicle's viscera coefficient in the male mice, the experimental data were analyzed by the methods of χ2 test and variance analysis.

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

toxicity to reproduction / fertility

Test material

Test material information

Constituent 1

Reference substance name:

Methyl methacrylate

EC Number:

201-297-1

EC Name:

Methyl methacrylate

Cas Number:

80-62-6

Molecular formula:

C5H8O2

IUPAC Name:

methyl 2-methylprop-2-enoate

Test material form:

liquid

Test animals

Species:

mouse

Strain:

other: Kunming

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Vehicle:

not specified

Details on exposure:

static inhalative exposure in a 50 L chamber

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

2 h

Frequency of treatment:

daily on 5 consecutive days

Post exposure period:

30 days since the 1st treatment

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

6.55 mg/L air

Remarks:

corresponds to 50% of the LC50

Doses / concentrations 2

Dose / conc.:

13.1 mg/L air

Remarks:

corresponds to 100% of the LC50

Doses / concentrations 3

Dose / conc.:

26.2 mg/L air

Remarks:

corresponds to 200% of the LC50

No. of animals per sex per dose:

5

Control animals:

yes

Examinations

Positive control:

cyclophosphamide 30 mg/kg administered by intraperitoneal injection

Results and discussion

Details on results:

Clinical signs: Severe clinical signs were observed; Low dose = 50% LC50: commotion, fleeing, jumping, grasping at the walls; Mid dose = 100% LC50: decreased movement, hobbling gait or even lateral lying, occasional tumultuous jumping; high dose = 200% LC50: Appearance of fluffy and unclean dorsal hairs, significantly reduced activity, aggregation lateral laying with reduced breathing

Sperm deformity rates were 1.80%, 2.46 and 2.58%, respectively, for the treatment groups and 1.42% in the for negative control group and 3.72% in the positive control group. Medium and high dose groups: the rate of sperm deformity in mice was significantly higher than that in the negative control group, with a uniform difference of (P<0.01); while the sperm deformity rate in mice in the low-dose group compared with the negative control group, the difference was not statistically significant (P>0.05).
Sperm deformities due to MMA are predominantly head deformities.

Testicular organ weight coefficients were comparable to the negative control.
Seminal vesicle gland organ weight coefficient of the high dose group was significantly different to the negative control group (P<0.05).