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Diss Factsheets

Toxicological information

Exposure related observations in humans: other data

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Administrative data

exposure-related observations in humans: other data
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
Occupational exposure of dental technicians to methyl methacrylate: Genotoxicity assessment
Soykut B, Erdem O, Yalcin CÖ, Üstündag A, Duydu Y, Akay C, Piskin B
Bibliographic source:
Mutat Res Gen Tox En 852 (2020) 503159, 1-5

Materials and methods

Test guideline
no guideline followed

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl methacrylate
EC Number:
EC Name:
Methyl methacrylate
Cas Number:
Molecular formula:
methyl 2-methylprop-2-enoate
Test material form:


Ethical approval:
confirmed, but no further information available
Details on study design:
For the genotoxicity tests, blood samples (5 ml) were collected in sodium heparin (BD Vacutainer, 367876, USA), lithium heparin (BD Vacutainer, 368884, USA), and EDTA- coated trace element (BD Vacutainer, 368381, USA) tubes via disposable intake sets (BD
Vacutainer, Eclipse 368609, USA). Preparations for the tests were initiated immediately after blood samples were taken.

Test groups:
Age (Mean±S.D.): 35.2±9.8
Duration of Work (Mean±S.D.): 16.1±9.1
Men: 61 (% 88.4)
Women: 8 (% 11.6)
Smoker: 40 (% 58)
Non-Smoker: 29 (% 42)

Control group:
Age (Mean±S.D.): 35.7±8.6
Duration of Work (Mean±S.D.): -
Men: 45 (% 67.2)
Women: 22 (% 32.8)
Smoker: 23 (% 34.3)
Non-Smoker: 44 (% 65.7)
Exposure assessment:
Details on exposure:
MMA levels in the ambient air of prosthesis laboratories were measured via sampling pump kit and Gastec Detector Tubes No.149 (Japan). Sampling was performed twice (before and after sample collection) in each of six different dental labs.
The detection limit was 1 ppm and the sampling volume was 200 ml.
The duration of each sampling was 4 min.
MMA levels in the ambient air of dental prosthesis laboratories were in the 25–50 ppm range, not exceeding international safety limits

Results and discussion

Comet tail intensity (%)
Test group: Mean = 4,8882
Control group: Mean= 4,75572
p:> 0.005

Applicant's summary and conclusion