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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994-07-20 until 1994-08-03
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
Principles of method if other than guideline:
NA
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phosphonobutane-1,2,4-tricarboxylic acid
EC Number:
253-733-5
EC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Cas Number:
37971-36-1
Molecular formula:
C7H11O9P
IUPAC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Details on test material:
- Physical state: Clear liquid
- Analytical purity: 39.7% (water content: 50.9%; and small amounts of some other compounds)
- Impurities (identity and concentrations): NA
- Purity test date: 19.10.95
- Lot/batch No.: 207
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
NA

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
The substrate (69% fine quartz sand, 10% peat, 20% kaolin, around 1% calcium carbonate) was first mixed dry in a plough-share mixer, and moistened with some of the final volume of water required. For the study, 2.5 g of the test substance were added to 500 mL deionized water and stirred on a magnetic stirrer for 30 min. The concentrations for the study were prepared by mixing equivalent parts of this stock solutions into the test substrate thoroughly with a domestic mixer. At the same time the rest of the deionised water required was mixed into the test substrate in each test container. It was claimed in the report that it was shown in a separate study, using 14C-labeled ingredients, under GLP-guidelines that the described method gives an homogenous and reproducible distribution of substances in the test substrate. The preparation of the artificial soil with the test substance is in agreement with the OECD 207 guideline.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Eisenia fetida andrei.
- Source: Prof. Graff, Forschungsanstalt für Landwirtschaft, Braunschweig
- Age at test initiation (mean and range, SD): More than two month old.
- Weight at test initiation (mean and range, SD): A mean weight of 0.38 g.
ACCLIMATION
- Acclimation period: One day prior to the start of the study.
- Acclimation conditions (same as test or not): Same of the test without the test substance.
- Health during acclimation (any mortality observed): NA

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Remarks:
NA
Post exposure observation period:
NA

Test conditions

Test temperature:
20 plus minus 1 °C.
pH:
At the beginning of the study the measured pH was 6.37 and 6.43. At the end of the study the pH value was 6.44.
It is indicated in study that it was shown in a separate study that the pH of the treated substrate (Bayhibit AM in freshly prepared soil) was not significantly different to control substrate.
Moisture:
Water content of the substrate (%) was 26.0 at the start of the study and 32.9 and 32.7 at the end of the study.
Water content as % of the maximum water capacity was 64.1 and 63.0 at the start of the study. When the test substance was added, 100 mL deionised water was also added to each test container so that the water content in the test substrate was around 35% when the worms were introduced.
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 1.5 litre preserving jars, covered with glass lids.
- Amount of soil or substrate: 500 g dry weight (equivalent to 775g wet weight) was prepared for each test container.
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: The vehicle is water and therefore vehicle control is the negative control. No additional 4 replicates vehicle control were needed.
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
its an artificial soil- 69% fine quartz sand, 10% dried finely ground peat, 20% kaolin, around 1% calcium carbonate.
OTHER TEST CONDITIONS
- Photoperiod: Constant light
- Light intensity: NA
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The number of surviving earthworms was counted 7 days after the start of the study. After 14 days, the weight and the number of surviving earthworms were determined.
VEHICLE CONTROL PERFORMED: See above (No. of replicates per control)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: NA
- Results used to determine the conditions for the definitive study: NA



Nominal and measured concentrations:
Nominal concentrations.: 1, 10, 100, 1000 mg/kg of Bayhibit AM in dry weight substrate (artificial soil consisting of sand, clay mineral and peat)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Mortality, weight changes, abnormalities like changes in the behaviour.
Remarks on result:
other: No remark
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Mortality, weight changes, abnormalities like changes in the behaviour.
Remarks on result:
other: No remark
Duration:
14 d
Dose descriptor:
LOEC
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Mortality, weight changes, abnormalities like changes in the behaviour.
Remarks on result:
other: LOEC = LLC
Details on results:
- Mortality at end of exposure period: No
- Total mass of adults at beginning of test: NA
- Changes in body weight of live adults (% of initial weight) at end of exposure period: Not significant

- Morphological abnormalities: No
- Behavioural abnormalities: No
Results with reference substance (positive control):
NA
Reported statistics and error estimates:
The weight results of the control and the test concentrations were analysed by U-test and were found not to differ significantly (P=0.05).

Any other information on results incl. tables

No remarks.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50, LLC, LOEC, TEC, NOEC are determinated,- see in executive summary.
Not harmful to earthworms at concentration lower or equal to 1000 mg/kg dry weight substrate.
Executive summary:

Different concentrations (1 -1000 mg) of Bayhibit AM, thoroughly mixed in artificial soil, were tested for toxicity of earthworms. After 14 days of exposure to the test material, no significant difference was observed in mortality rate and in weight in any of the different concentration groups that were examined compare to the control group. In addition, abnormalities, like changes in behaviour were also not observed in the study. Thereby, the LC50 (14 days) was determined for Bayhibit AM as > 1000 mg/kg dry weight substrate. In relation to weight alteration and symptoms, the no-observed-effect concentration (NOEC) was 1000 mg/kg dry weight substrate, the lowest-observed-effect concentration (LOEC) and the lowest tested concentration with mortality (LLC) was > 1000 mg/kg dry weight substrate.

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