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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-11-03 to 1993-11-17
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Principles of method if other than guideline:
Duration: 14 days instead of 96 hours.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phosphonobutane-1,2,4-tricarboxylic acid
EC Number:
253-733-5
EC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Cas Number:
37971-36-1
Molecular formula:
C7H11O9P
IUPAC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Details on test material:
- name of the test material: BAYHIBIT AM
- Composition of test material, percentage of components: 39.66% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid
- Lot/batch No.: 207
- Sample no / year. 342702/1991
-Expiration date of lot/batch: 1995-10-19 (prolonged)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
NA

Test solutions

Vehicle:
no
Details on test solutions:
NA

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Danio rerio; Zebra danio
- Source: West-Aquarium (Bad Lauterberg; Germany)
- Length: 2.5 - 3.5 cm
- Mortality during pre-exposure to synthetic fresh water according to ISO: < 2%
- Medical pre-treatment: no

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
14 d
Post exposure observation period:
NA (The fishes were scarified after 14 days of exposure and then the length and bodyweight were determined).

Test conditions

Hardness:
12.8 - 15.4°dH
Test temperature:
ca. 21°C (+/-2°C)
pH:
7.8 mg/L - 9.6 mg/L
Dissolved oxygen:
7.8 mg/L - 9.6 mg/L
Salinity:
according to ISO
Nominal and measured concentrations:
Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-12; 30-34; 100-109; 309-337; 994-1086
Details on test conditions:
TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Test medium: 5 L, aerated
- Vehicle: no
- No. of organisms per vessel and concentration: 10
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 042 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: No remarks
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 042 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: No remarks
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 042 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: No remarks
Details on results:

A LC50 (96h) of > 1042 mg/L was derived from the available prolonged toxicity study in fish over 14 days (result: LC50 (21d) > 1042 mg/L).
No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls.
Results with reference substance (positive control):
NA
Reported statistics and error estimates:
NA

Any other information on results incl. tables

Sublethal observations / clinical signs:

No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A LC50 (96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L).
Executive summary:

A LC50(96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish ( species: zebra danio) over 14 days. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L). No further testing (in respect of classification and labelling etc.) is required.

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