Registration Dossier
Registration Dossier
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EC number: 253-733-5 | CAS number: 37971-36-1
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-11-03 to 1993-11-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- yes
- Remarks:
- LC50 after 96 hours determined
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- NA
- Vehicle:
- no
- Details on test solutions:
- NA
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio; Zebra danio
- Source: West-Aquarium (Bad Lauterberg; Germany)
- Length: 2.5 - 3.5 cm
- Mortality during pre-exposure to synthetic fresh water according to ISO: < 2%
- Medical pre-treatment: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- NA (The fishes were scarified after 14 days of exposure and then the length and bodyweight were determined).
- Hardness:
- 12.8 - 15.4°dH
- Test temperature:
- ca. 21°C (+/-2°C)
- pH:
- 7.8 mg/L - 9.6 mg/L
- Dissolved oxygen:
- 7.8 mg/L - 9.6 mg/L
- Salinity:
- according to ISO
- Nominal and measured concentrations:
- Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-12; 30-34; 100-109; 309-337; 994-1086 - Details on test conditions:
- TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Test medium: 5 L, aerated
- Vehicle: no
- No. of organisms per vessel and concentration: 10 - Reference substance (positive control):
- no
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 042 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: No remarks
- Details on results:
- A LC50 (96h) of > 1042 mg/L was derived from the available prolonged toxicity study in fish over 14 days (result: LC50 (21d) > 1042 mg/L).
No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls. - Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- NA
- Sublethal observations / clinical signs:
No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A LC50 (96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L).
- Executive summary:
A LC50(96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish ( species: zebra danio) over 14 days. Thus, the LC50(96h) is higher than the concentration of the limit test (100 mg/L). No further testing (in respect of classification and labelling etc.) is required.
Reference
Description of key information
A LC50 (96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 1 042 mg/L
Additional information
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