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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-11-03 to 1993-11-24
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
yes
Principles of method if other than guideline:
Compared with OECD 211, adopted in 2008): the light intensity was slightly too high (25 instead of max. 20 µE/m2*s).
TOC/ COD was not determined.
CSB was determined.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phosphonobutane-1,2,4-tricarboxylic acid
EC Number:
253-733-5
EC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Cas Number:
37971-36-1
Molecular formula:
C7H11O9P
IUPAC Name:
2-phosphonobutane-1,2,4-tricarboxylic acid
Details on test material:
- name of the test material: BAYHIBIT AM
- Composition of test material, percentage of components: 39.66% (w/w) 2-phosphonobutane-1,2,4-tricarboxylic acid
- Lot/batch No.: 207
- Sample no / year. 342702/1991
- Expiration date of lot/batch: 1995-10-19 (prolonged)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium.

Test solutions

Vehicle:
no
Details on test solutions:
NA

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
NA

Test conditions

Hardness:
Not indicated
Test temperature:
ca. 20°C +/-2°C; per single test: 20°C +/-1°C
pH:
Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6
Dissolved oxygen:
Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1
Salinity:
Not indicated
Nominal and measured concentrations:
Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159
Details on test conditions:
TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus"

Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 1 071 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
immobilisation
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 329 - < 1 071 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
104 mg/L
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: DUNNETT-TEST
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
329 mg/L
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: DUNNETT-TEST
Details on results:
NA
Results with reference substance (positive control):
NA
Reported statistics and error estimates:
The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).

Any other information on results incl. tables

No remarks.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L
Executive summary:

A prolonged toxicity study in Daphnia magna was performed over 21 days.

The respective effects on reproduction (NOEC, LOEC, EC50) and mobility were determined (EC50):

EC 50 (immobilisation, 21d): > 1071 mg/L

EC 50 (reproduction, 21d): > 329 - < 1071 mg/L

NOEC (reproduction, 21d): 104 mg/L

LOEC (reproduction, 21d): 329 mg/L