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EC number: 253-733-5 | CAS number: 37971-36-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- NA
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- Principles of method if other than guideline:
- NA
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: effluent from a sewage treatment plant
- Details on inoculum:
- Culture medium was inoculated by the effluent from a sewage treatment plant (5 mL/2000 mL).
- Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- The ony carbon source was 2-phosphonobutane-1,2,4-tricarboxylic acid (10 mg C/L).
- Reference substance:
- aniline
- Preliminary study:
- NA
- Test performance:
- NA
- Parameter:
- % degradation (DOC removal)
- Remarks on result:
- not determinable
- Details on results:
- Under test conditions no biodegradation was observed. Percentage degradation is not indicated.
- Results with reference substance:
- Nearly complete biodegradation after 7 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- 2-phosphonobutane-1,2,4-tricarboxylic acid was found not biodegradable in the "Modified OECD-Screening-Test".
- Executive summary:
2-phosphonobutane-1,2,4-tricarboxylic acid was examined for biodegradability according to the "Modified OECD-Screening-Test".
Under test conditions no biodegradation was observed.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- NA
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 A (Inherent Biodegradability: Modified SCAS Test)
- Deviations:
- no
- Principles of method if other than guideline:
- NA
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- NA
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- usual / no specific inoculum from a sewage treatment plant
- Duration of test (contact time):
- ca. 3 mo
- Initial conc.:
- 64 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- - pH = 2
- 64 mg 2-phosphonobutane-1,2,4-tricarboxylic acid /L is equivalent to 20 mg C/L
- inoculum: 3g/L dry matter
- no addition of 2-phosphonobutane-1,2,4-tricarboxylic acid at day 126 to 131 - Reference substance:
- not specified
- Preliminary study:
- NA
- Test performance:
- NA
- Parameter:
- % degradation (DOC removal)
- Remarks on result:
- other: no biodegradation was observed
- Details on results:
- - The result was evaluated by taken into account the concentration at the end of a cycle used as blank value for the following days (see below).
- Under test conditions no elimination was observed as the degradation is lower than 70% within 3 months.
- Within the first days of the test an apparent elimination of about 60% was observed. This effect was explained by adsorption to activated sludge.
- Sludge was sampled just before day 126 (first day without addition of 2-phosphonobutane-1,2,4-tricarboxylic acid) and microscopically analysed. A lot of microorganisms were adversely affected or dead.
- More realistic eliminations were evaluated when not the DOC values of controls but the DOC concentration at the end of a cycle was used next day as blank value because the degradation of organic compounds in controls and test vessels is not always comparable. - Results with reference substance:
- NA
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- 2-phosphonobutane-1,2,4-tricarboxylic acid was found not inherently biodegradable in the "Modified SCAS-Test".
- Executive summary:
2-phosphonobutane-1,2,4-tricarboxylic acid was examined for inherent biodegradability according to the "Modified SCAS-Test".
Under test conditions no biodegradation was observed.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- NA
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- Principles of method if other than guideline:
- NA
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: effluent from a sewage treatment plant
- Details on inoculum:
- Culture medium was inoculated by the effluent from a sewage treatment plant (5 mL/2000 mL).
- Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- 32 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- The only carbon source was 2-phosphonobutane-1,2,4-tricarboxylic acid (10 mg C/L).
- Reference substance:
- aniline
- Preliminary study:
- NA
- Test performance:
- NA
- Parameter:
- % degradation (DOC removal)
- Remarks on result:
- other: no biodegradation observed
- Details on results:
- Under test conditions no biodegradation was observed. Percentage degradation is not indicated.
- Results with reference substance:
- Nearly complete biodegradation after 7 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- 2-phosphonobutane-1,2,4-tricarboxylic acid was found not biodegradable in the "Modified OECD-Screening-Test".
- Executive summary:
2-phosphonobutane-1,2,4-tricarboxylic acid was examined for biodegradability according to the "Modified OECD-Screening-Test".
Under test conditions no biodegradation was observed.
Referenceopen allclose all
No remarks.
No remarks.
No remarks.
Description of key information
2-phosphonobutane-1,2,4-tricarboxylic acid was examined for biodegradability according to the "Modified OECD-Screening-Test" (Guideline 301 E) and according to the "Modified SCAS-Test as described in OECD Guideline 302 A. In both tests, no (inherent) biodegradation was observed under test conditions (Horstmann, Grohmann, 1988). Also no biodegradation was observed in the "Closed Bottle Test" according to OECD Guideline 301 D (Kästner, Gode, 1983). In another test 2-phosphonobutane-1,2,4-tricarboxylic acid tetra sodium salt was examined for inherent biodegradability according to the " Zahn-Wellens test". Again, no biodegradation was observed (BUA, 1994).
Thus, 2-phosphonobutane-1,2,4-tricarboxylic acid is not ready biodegradable in water.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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