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EC number: 939-464-2 | CAS number: 121617-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Bacteria toxicity testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 June 1992 - 01 July 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets generally accepted scientific principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
- Principles of method if other than guideline:
- Determination of the biological toxicity of substances hazardous in water to Pseudomonas putida according to Bringmann and Kühn (Bringmann, G. and Kühn, R.: Zeitschrift für Wasser- und Abwasser-Forschung 10 (1977) p. 87-98).
Principles:
The test organism (Pseudomonas putida) was incubated in a defined liquid growth medium with different concentrations of test substance over multiple generations at 24.8 - 25 °C during 18 ± 1 hours. The cell density was determined photometrically as the extinction of the cell suspension at 436 nm. The inhibition of cell growth in presence of test substance compared to that of the control was derived grahically from the extinction values measured at the end of the incubation period. - GLP compliance:
- yes
Test material
- Reference substance name:
- Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with triethanolamine
- EC Number:
- 939-464-2
- Cas Number:
- 121617-08-1
- Molecular formula:
- C6 H15 N O3 . C6 H6 O3 S
- IUPAC Name:
- Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with triethanolamine
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): MARLOPON AT 50
- Physical state: yellow-brown liquid
- Analytical purity: 56.2% Benzenesulfonic acid, C10-13-alkyl derivs., compds. with triethanolamine (CAS no. 68411-31-4) (of which 92% alkylbenzolsulfonate-triethanolamine salt)
- CAS no.: 68411-31-4
- Lot/batch No.: 621
- Production date of the lot/batch:1/92
- Expiration date of the lot/batch:1/93
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- The extinction of bacterial inoculum was measured using a Zeiss-Photometer PM 6 at a wavelength of 436 nm, and diluted with NaCl-solution to a extinction value of 0.300 (± 5%) before inoculation of the test solutions.
Study design
- Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 18 h
- Post exposure observation period:
- None
Test conditions
- Test temperature:
- 24.8 - 25.0 °C
- pH:
- pH of the different test substance control dilutions (no bacteria added) without the nutrient solutions: 6-6.8
pH of the different test substance control dilutions (no bacteria added) with the nutrient solutions after 18 hours of incubation: 6.8 - Nominal and measured concentrations:
- 0, 50, 100, 200 and 400 mg MARLOPON AT 50/L
which is equal to: 0, 28, 56, 112 and 225 mg Benzenesulfonic acid, C10-13-alkyl derivs., compds. with triethanolamine (CAS no. 68411-31-4)/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlymeyer flasks
- Type (delete if not applicable): closed with cellulose plugs
- Fill volume: 100 mL
- Aeration: not reported
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
- Biomass loading rate: The extiction (at 436 nm) of the bacterial inoculum was corrected with NaCl solution to 0.300 (± 5%) before addition to the test solutions
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the test substance was diluted in bidest.
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cell density by measurement of extinction at 436 nm after 18 hours of incubation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: factor 2
- Range finding study
- Test concentrations: 0, 100, 200, 400 and 800 mg MARLOPON AT 50/ml
- Results used to determine the conditions for the definitive study: the lowest tested concentration gave a 20% reduction compared to the control, all higher test concentrations gave a 85% reduction compared to the control. As the ojective was to derive a EC10, it was decide to repeat the test in which one more lower concentration (50 mg MARLOPON AT 50/L) was tested in the main test. This main test was done in duplicate. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: product (MARLOPON AT 50)
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: CAS no. 68411-31-4
- Basis for effect:
- growth inhibition
- Details on results:
- The results for the pilot and two main experiments are given in the section 'any other information on results incl. tables' below. The EC10 derived grahpically from the extinction values obtained in the main experiment A and B were 110 and 84 mg MARLOPON AT 50/L, giving an average EC10 of 92 mg MARLOPON AT 50/L. This can be recalculated to 92 * 0.562 = 55 mg Benzenesulfonic acid, C10-13-alkyl derivs., compds. with triethanolamine (CAS no. 68411-31-4)/L.
Any other information on results incl. tables
Table 1. Pilot experiment: Effect of MARLOPON AT 50 on the growth of Pseudomonas putida after 18 hours of incubation, measured photometrical as extinction at 436 nm.
Concentration MARLOPON AT 50 (mg/L) |
Extinction (436 nm) |
|||||
Replica 1 |
Replica 2 |
Replica 3 |
Replica 4 |
Replica 5 |
Mean |
|
0 |
480 |
470 |
487 |
467 |
492 |
479 |
100 |
380 |
382 |
388 |
|
|
383 |
200 |
60 |
63 |
61 |
|
|
61 |
400 |
69 |
66 |
63 |
|
|
66 |
800 |
65 |
67 |
67 |
|
|
66 |
Table 2. Main experiment A: Effect of MARLOPON AT 50 on the growth of Pseudomonas putida after 18 hours of incubation, measured photometrical as extinction at 436 nm.
Concentration MARLOPON AT 50 (mg/L) |
Extinction (436 nm) |
|||||
Replica 1 |
Replica 2 |
Replica 3 |
Replica 4 |
Replica 5 |
Mean |
|
0 |
478 |
405 |
404 |
407 |
387 |
416 |
50 |
486 |
485 |
465 |
|
|
479 |
100 |
400 |
394 |
399 |
|
|
398 |
200 |
61 |
61 |
62 |
|
|
61 |
400 |
66 |
70 |
68 |
|
|
68 |
Table 3. Main experiment B: Effect of MARLOPON AT 50 on the growth of Pseudomonas putida after 18 hours of incubation, measured photometrical as extinction at 436 nm.
Concentration MARLOPON AT 50 (mg/L) |
Extinction (436 nm) |
|||||
Replica 1 |
Replica 2 |
Replica 3 |
Replica 4 |
Replica 5 |
Mean |
|
0 |
512 |
571 |
476 |
517 |
492 |
514 |
50 |
508 |
502 |
536 |
|
|
515 |
100 |
421 |
436 |
439 |
|
|
432 |
200 |
68 |
70 |
68 |
|
|
69 |
400 |
74 |
77 |
76 |
|
|
76 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 18 h EC10 for Pseudomonas putida was 55 mg/L, based on growth inhibition.
- Executive summary:
The test organism (Pseudomonas putida) was incubated in a defined liquid growth medium with different concentrations of test substance over multiple generations at 24.8 - 25 °C during 18 ± 1 hours. The following concentrations were tested: 0, 50, 100, 200 and 400 mg MARLOPON AT 50/L which is equal to: 0, 28, 56, 112 and 225 mg Benzenesulfonic acid, C10-13-alkyl derivs., compds. with triethanolamine (CAS no. 68411-31-4)/L.
After 18 hours, the cell density was determined photometrically as the extinction of the cell suspension at 436 nm. The inhibition of cell growth in presence of test substance compared to that of the control was derived grahically from the extinction values measured at the end of the incubation period.
The 18 h EC10 for Pseudomonas putida was 92 mg MARLOPON AT 50/ ml which is equal to 55 mg Benzenesulfonic acid, C10-13-alkyl derivs., compds. with triethanolamine (CAS no. 68411-31-4)/L based on growth inhibition.
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