Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with triethanolamine

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to terrestrial arthropods: long-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well reported published study.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Effects and risk assessment of linear alkylbenzene sulfonates in agriculture soil. 5. Probabilistic risk assessment of linear alkylbenzene sulfonates in sludge-amended soils.

Author:

Jensen, J., Lokke, H., Holmstrup, M., Krogh, P.H. and Elsgaard, L.

Year:

2001

Bibliographic source:

Environ. Toxicol. Chem. 20:1690-1697

Reference 2

Reference Type:

review article or handbook

Title:

Tests on the Oribatid Mite Platynothrus peltifer

Author:

van Gestel, C, Doornekamp, A

Year:

1998

Bibliographic source:

In: Handbook of Soil Invertebrate Toxicity Tests, pp. 113-130. Lokke, H, and van Gestel, C, eds. John Wiley and Sons, New York.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Groups of 20 P. Peltifer mites were exposed to various concentrations of LAS up to 1000 mg/kg dw soil. The exposure lasted 10 weeks. The mortality and reproduction of the mites at the end of this period was assessed.

GLP compliance:

not specified

Application method:

soil

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

C16-19 H25-31 Na O3 S

IUPAC Name:

sodium 4-undecylbenzenesulfonate

Details on test material:

- Name of test material (as cited in study report): LAS (Marlon A 350)

Sampling and analysis

Analytical monitoring:

not specified

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil: Test substance was dissolved in a suitable amount of water and thoroughly mixed into test substrate.
- Controls: Controls were treated similarly to test containers, but without test substance.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water

Test organisms

Test organisms (species):

other: Platynothrus peltifer

Animal group:

other: soil-dwelling

Details on test organisms:

TEST ORGANISM
- Common name: oribatid mite
- Source: Needle litter from coniferous forest near Roggebotszand, Netherlands
- Age at test initiation (mean and range, SD): adult
- Date of collection: 1993

ACCLIMATION
- Acclimation period: animals used were adults collected as tritonymphs
- Acclimation conditions (same as test or not): Culture pots contained a 1-2 cm layer of plaster of Paris and covered with cheese cloth.
- Feeding: Green algae on smalle pieces of bark from pine twigs.

Study design

Study type:

laboratory study

Total exposure duration:

10 wk

Test conditions

Test temperature:

18-20 degree C

pH (if soil or dung study):

6.0 +/- 0.5

Humidity:

Water content of soil was 30-40% of total water capacity.

Photoperiod and lighting:

12 hrs light/12 hrs dark, ~400 lux

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 5 cm diameter x 3.5 cm high containers. Bottom consists of a 1 mm mesh sealed with plastic foil. The top is covered with a hole covered with cheese cloth to allow aeration.
- Amount of soil or substrate: 75 g (dry weight)
- No. of organisms per container (treatment): 20
- No. of replicates per treatment group: 5
- No. of replicates per control: 1

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil): Lufa 2.2
- % clay: 5.1%
- Organic carbon (%): 3.9%

OTHER TEST CONDITIONS
- Photoperiod: 12 hrs light/12 hrs dark
- Light intensity: ~400 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : At 2, 6, and 12 weeks after start of exposure, the mites are removed with a Tullgren apparatus and counted. The percentage mortality and number of offspring produced.

Nominal and measured concentrations:

0, 1, 10, 100, 1000 mg/kg dry soil

Reference substance (positive control):

yes

Remarks:

copper chloride

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

10 wk

Dose descriptor:

NOEC

Effect conc.:

320 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: Platynothrus peltifer

Effect concentrations 2

Duration:

10 wk

Dose descriptor:

LOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

The NOEC in LUFA 2.2 soil was 320 mg/kg soil dw based on reproduction.

Any other information on results incl. tables

                                  

Species	Endpoint	NOEC	EC10	EC50
Eisenia foetida	Reproduction		383	558
Lumbricus terrestris	Weight	667
Aporrectodea caliginosa	Reproduction		14	129
Aporrectodea longa	Reproduction		27	137
Folsomia fimetaria	Reproduction		96	442
Folsomia candida	Reproduction		18	91
Hypoaspis aculeifer	Reproduction		81.7	236
Enchytraeus albidus	Reproduction		6.2	40.5
Platynothrus peltifer	Reproduction	320		467
Isotoma viridis	Growth		41
Hypogastrura assimilis	Reproduction		99.8	421

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC for P. peltifer was 320 mg/kg.

Executive summary:

Groups of 20 P. Peltifer mites were exposed to various concentrations of LAS up to 1000 mg/kg dw soil. The exposure lasted 10 weeks. The mortality and reproduction of the mites at the end of this period was assessed. The NOEC for P. peltifer, based on reproduction was 320 mg/kg dw soil. The LOEC was 1000 mg/kg dw soil.