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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well reported published study.
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Effects and risk assessment of linear alkylbenzene sulfonates in agriculture soil. 5. Probabilistic risk assessment of linear alkylbenzene sulfonates in sludge-amended soils.
Author:
Jensen, J., Lokke, H., Holmstrup, M., Krogh, P.H. and Elsgaard, L.
Year:
2001
Bibliographic source:
Environ. Toxicol. Chem. 20:1690-1697
Reference Type:
review article or handbook
Title:
Tests on the Oribatid Mite Platynothrus peltifer
Author:
van Gestel, C, Doornekamp, A
Year:
1998
Bibliographic source:
In: Handbook of Soil Invertebrate Toxicity Tests, pp. 113-130. Lokke, H, and van Gestel, C, eds. John Wiley and Sons, New York.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Groups of 20 P. Peltifer mites were exposed to various concentrations of LAS up to 1000 mg/kg dw soil. The exposure lasted 10 weeks. The mortality and reproduction of the mites at the end of this period was assessed.
GLP compliance:
not specified
Application method:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): LAS (Marlon A 350)

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Method of mixing into soil: Test substance was dissolved in a suitable amount of water and thoroughly mixed into test substrate.
- Controls: Controls were treated similarly to test containers, but without test substance.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water

Test organisms

Test organisms (species):
other: Platynothrus peltifer
Animal group:
other: soil-dwelling
Details on test organisms:
TEST ORGANISM
- Common name: oribatid mite
- Source: Needle litter from coniferous forest near Roggebotszand, Netherlands
- Age at test initiation (mean and range, SD): adult
- Date of collection: 1993

ACCLIMATION
- Acclimation period: animals used were adults collected as tritonymphs
- Acclimation conditions (same as test or not): Culture pots contained a 1-2 cm layer of plaster of Paris and covered with cheese cloth.
- Feeding: Green algae on smalle pieces of bark from pine twigs.

Study design

Study type:
laboratory study
Total exposure duration:
10 wk

Test conditions

Test temperature:
18-20 degree C
pH (if soil or dung study):
6.0 +/- 0.5
Humidity:
Water content of soil was 30-40% of total water capacity.
Photoperiod and lighting:
12 hrs light/12 hrs dark, ~400 lux
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 5 cm diameter x 3.5 cm high containers. Bottom consists of a 1 mm mesh sealed with plastic foil. The top is covered with a hole covered with cheese cloth to allow aeration.
- Amount of soil or substrate: 75 g (dry weight)
- No. of organisms per container (treatment): 20
- No. of replicates per treatment group: 5
- No. of replicates per control: 1

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil): Lufa 2.2
- % clay: 5.1%
- Organic carbon (%): 3.9%

OTHER TEST CONDITIONS
- Photoperiod: 12 hrs light/12 hrs dark
- Light intensity: ~400 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : At 2, 6, and 12 weeks after start of exposure, the mites are removed with a Tullgren apparatus and counted. The percentage mortality and number of offspring produced.
Nominal and measured concentrations:
0, 1, 10, 100, 1000 mg/kg dry soil
Reference substance (positive control):
yes
Remarks:
copper chloride

Results and discussion

Effect concentrationsopen allclose all
Duration:
10 wk
Dose descriptor:
NOEC
Effect conc.:
320 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Platynothrus peltifer
Duration:
10 wk
Dose descriptor:
LOEC
Effect conc.:
1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
The NOEC in LUFA 2.2 soil was 320 mg/kg soil dw based on reproduction.

Any other information on results incl. tables

                                  

Species

Endpoint

NOEC

EC10

EC50

Eisenia foetida

Reproduction

383

558

Lumbricus terrestris

Weight

667

Aporrectodea caliginosa

Reproduction

14

129

Aporrectodea longa

Reproduction

27

137

Folsomia fimetaria

Reproduction

96

442

Folsomia candida

Reproduction

18

91

Hypoaspis aculeifer

Reproduction

81.7

236

Enchytraeus albidus

Reproduction

6.2

40.5

Platynothrus peltifer

Reproduction

320

467

Isotoma viridis

Growth

41

Hypogastrura assimilis

Reproduction

99.8

421

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC for P. peltifer was 320 mg/kg.
Executive summary:

Groups of 20 P. Peltifer mites were exposed to various concentrations of LAS up to 1000 mg/kg dw soil. The exposure lasted 10 weeks. The mortality and reproduction of the mites at the end of this period was assessed. The NOEC for P. peltifer, based on reproduction was 320 mg/kg dw soil. The LOEC was 1000 mg/kg dw soil.