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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well-documented, peer-reviewed publication.

Data source

Reference
Reference Type:
publication
Title:
Effects of an Anionic Surfactant, Linear Alkylbenzene Sulfonate, on Survival, Reproduction and Growth of the Soil-Living Collembolan Folsomia Fimetaria
Author:
Holmstrup, M, and Krogh, PH
Year:
1996
Bibliographic source:
Environmental Toxicology and Chemistry, Vol. 15, No. 10, 1745-1748

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted.
GLP compliance:
no
Application method:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Marlon A 350
- Molecular weight (if other than submission substance): 344 g/mol mean molecular weight
- Analytical purity: 50% active substance
- Composition of test material, percentage of components: average chain length = 11.53

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples were taken 2, 17, and 31 days after the soil was spiked.

Test substrate

Details on preparation and application of test substrate:
LAS was dissolved in water and mixed with 30 g moist soil (25.5 g dry). The mixture was prepared immediately before the experiment.

Test organisms

Test organisms (species):
Folsomia sp.
Animal group:
Collembola (soil-dwelling springtail)
Details on test organisms:
TEST ORGANISM
- Source: laboratory culture
- Age at test initiation (mean and range, SD): 23-26 days
- Weight at test initiation (mean and range, SD):

Study design

Study type:
laboratory study
Total exposure duration:
21 d

Test conditions

Test temperature:
20 degrees C
pH (if soil or dung study):
5.5
Photoperiod and lighting:
12 hrs light/12 hrs dark
Details on test conditions:
TEST SYSTEM
- Amount of soil or substrate: 30 g moist
- No. of organisms per container (treatment): 10 male/10 female
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Speyer, Germany
- Soil texture (if natural soil)
- % sand: 34.8
- % silt: 5.6
- % clay: 5.2
- % gravel: 54.6
- Organic carbon (%): 2.3
- Pretreatment of soil: dried at 80 degrees C
- Storage (condition, duration): 5 degrees C

OTHER TEST CONDITIONS
- Photoperiod: 12/12 hrs

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : survival and reproduction at 21 days of exposure measured using digital image processing
Nominal and measured concentrations:
nominal: 0-1000 mg/kg dry soil

Results and discussion

Effect concentrations
Key result
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
147 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
reproduction
Remarks on result:
other: 116-178
Details on results:
Adult survival was unaffected at all concentrations. Reproductive output decreased linearly with increasing concentration, with 430 juveniles produced in control containers, to only 150 juveniles produced at the highest concentration (1000 mg/kg dw soil).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 21-day EC10 for F. fimetaria was 147 mg/kg dw soil based on reproduction.
Executive summary:

Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction. The EC10 based on reproduction was 147 mg/kg dw soil.