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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
April 23, 1987-June 8, 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: read-across to Klimisch 1 study (well-documented GLP study)

Data source

Referenceopen allclose all

Reference Type:
other company data
Title:
Unnamed
Year:
1987
Report Date:
1987
Reference Type:
other company data
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
yes
Remarks:
% particles less than 20 µg not given; 4.1% instead of maximum 3% organic carbon in the test soil; sand was sieved with 1 mm mesh instead of 0.5 mm mesh; weight and variability of seeds not reported.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
not specified
Details on preparation and application of test substrate:
The test was conducted in an artificial soil consisting of commercially available potting compost (WC-B) and washed, sieved (1 mm mesh), dried (temperature and time not reported) silver sand (1:9 potting soil:washed sand).

Test organisms

open allclose all
Species:
Sorghum bicolor
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: sorghum
- Variety: Dekalb 64
- Source of seed: Dekalk, USA
- Historical germination of seed (germination of seed lot tested): viability of at least 80%
Species:
Helianthus annuus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: sunflower
- Variety: Rodeo
- Source of seed: Codasal, France
- Historical germination of seed (germination of seed lot tested): viability of at least 80%
Species:
Phaseolus aureus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: mung bean
- Source of seed: Clause Seeds, UK
- Historical germination of seed (germination of seed lot tested): viability of at least 80%

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
11-37 degrees C
pH:
6.8-7.9
Moisture:
11.9%
Details on test conditions:
TEST SYSTEM
- Testing facility: Jealott's Hill Greenhouse Number 130/2
- Test container (type, material, size): 10-cm diameter, non-porous plastic plant pots, capacity of approx. 600 g
- Amount of soil: ~600 g
- No. of seeds per container: 8
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

GROWTH CONDITIONS
- Photoperiod: at least 14 hrs
- Light source: if natural sunlight was poor, mercury vapor lamps were used
- Light intensity and quality: 5,000 lux
- Watering regime and schedules: capillary matting sub-irrigation system, twice daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : seedling emergence
Nominal and measured concentrations:
0, 1, 10, 100, 1000 mg/kg active ingredient in dry soil
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Species:
other: all 3
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Sorghum bicolor
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
167 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
289 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Phaseolus aureus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
316 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Sorghum bicolor
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
68 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
116 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth
Species:
Phaseolus aureus
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
126 mg/kg soil dw
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
growth
Details on results:
There was 91% emergence of sunflowers at all concentrations. Mung bean emergence was at least 84% at concentrations of 100 mg/kg AI or below. At 1000 mg/kg AI, emergence was only 75%. Seed emergence was 78-84% in sorghum for controls and all concentrations but the highest. At 1,000 mg/kg AI, emergence was only 69%. At the 1000 mg/kg AI concentration, sunflowers and mung beans barely emerged, and showed signs of dying. Sorghum shoots at this concentration were healthy but stunted. The low emergence in mung beans may be due to a cold spell early in the test.

Any other information on results incl. tables

Number (Percent) of Plants Emerged at Day 7

Species

Control

1 mg/kg

10 mg/kg

100 mg/kg

1000 mg/kg

Sorghum

26 (81)

27 (84)

27 (84)

25 (78)

22 (69)

Sunflower

30 (94)

32 (100)

30 (94)

32 (100)

29 (91)

Mung bean

31 (97)

27 (84)

30 (94)

27 (84)

24 (75)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC for all three species is 100 mg/kg AI. The 21-day EC50 for sunflower was 289 mg/kg AI, for sorghum it was 167 mg/kg AI, and for mung bean it was 316 mg/kg AI.
Executive summary:

The toxicity of the test substance to three species of plant, sunflower, sorghum, and mung bean, was determined. Seeds of these three species were grown in soil containing concentrations of the test ingredient of 0, 1, 10, 100, 1000 mg/kg active ingredient in dry soil. The emergence and growth of seedlings was monitored for 21 days. There was a decrease in emergence of all three species at the highest concentration, with some shoots barely emerging, and stunted and dying. The NOEC for all three species is 100 mg/kg AI. The 21-day EC50 for sunflower was 289 mg/kg AI, for sorghum it was 167 mg/kg AI, and for mung bean it was 316 mg/kg AI.