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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: APHA method (1980)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triethanolamine
- Analytical purity: 97 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- concentrations were analysed by gas-liquid chromatography (GLC)

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Test species: Fathead minnow (Pimephales promelas)      
- age (d): 30       
- mean body length (mm): 18.1 ± 0.788      
- mean weight (g): 0.083 ± 0.0083      
- loading (g/l): 0.1153

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
25.7 ± 0.38 °C
pH:
7.8 ± 0.07
Dissolved oxygen:
7.3 mg/l
Nominal and measured concentrations:
Nominal test concentrations (g/l): 0, 7.30, 11.2, 17.3, 26.6, and 40.9
test substance recovery by GLC: 107.2 ± 3.8% (n = 4)
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 fish/concentration
- tank volume (l): 1.0 (additions 14.4 v/d)
- pH: the pH of the stock solution (90.4 mg/l) was adjusted to that of lake water using HCl.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
11 800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Reported statistics and error estimates:
The estimated LC/EC50 values with corresponding 95% confidence intervals were calculated using the corrected average of the analyzed tank concentrations and the Trimmed Spearman-Kaerber method (Hamilton et al., 1977).

Any other information on results incl. tables

Effect values (related to measured concentrations): 96-h EC50 (g/l): 11.8 (10.6-13.0)

Mortalities

Nominal conc (g/l)

Mean measured (corr.)

No. of fish at start

Dead fish after

24h

48h

72h

96h

Control

< 0.02

20

0

0

0

0

7.30

6.14

20

0

0

0

0

11.2

9.86

20

1

3

4

4

17.3

15.4

20

15

17

18

18

26.6

24.4

20

20

20

20

20

40.9

38.1

20

20

20

20

20


Physiological/behavioral effects 
Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96 h LC50 was 11800 mg/L based on measured concentrations.
Executive summary:

Acute toxicity of TEA to fathead minnows was tested according to APHA method (1980). No mortaility was observed in the control.

Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.

Effect values (related to measured concentrations): 96-h LC50 (g/l): 11.8 (10.6-13.0)