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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Results of the harmful effects of water pollutants to Daphnia magna in the 21 day reproduction test.
Author:
Kuehn R, Pattard M, Pernak KD and Winter A
Year:
1989
Bibliographic source:
Water Research 23(4): 501-510.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German Federal Environmental Agency internal method, comparable to OECD TG 211.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triethanolamine
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
- dilution water: synthetic freshwater according to German Industrial Standard DIN 38412, Part 1 and Part 11 (1982): 11.76 g/l CaCl2*2H2O, 4.93 g/l MgSO4*7H2O, 2.59 g/l NaHCO3, 0.23 g/l KCl, oxygen-saturated, pH 8.0 ± 0.2 - stock solution of test substance: 2000 mg/l (dilution water heated to 30 °C)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- test animals: Daphnia magna (IRCHA strain)
- age: 24 h
- culture:       
- 20-30 animals in 40 2-l beakers filled with >= 1.6 l tap water (hardness: 285.76 mg/l as CaCO3; pH 7.6-7.7; oxygen-saturated)      
- temperature: 20 °C      
- fluorescent light (9 h light : 15 h dark)      
- offspring removed daily from cultures      
- feeding: dried algae (Scenedesmus sp.; 9 g/l, 2 ml/beaker)

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Nominal and measured concentrations:
nominal: 8, 16, 32, 64, 128, 256, 512, 1000 mg/L
Details on test conditions:
- test vessel: closed
- test concentration: 8-1000 mg/l, dilution ratio: 1:2
- replicates: 4/concentration, 1 animal/50 ml, 20 animals/conc.
- semistatic conditions (3 times/week)
- feeding: 3 times/week (Tetramin fish feed)
- illumination: fluorescent light (9 h light : 15 h dark)
- temperature: 25 ± 1 °C
- observations: 3 times/week: dead/immobile animals, offspring, pH, dissolved oxygen
- analytical monitoring of test substance concentration in stock solution, test vessels, and blanks
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
16 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 21 d NOEC was 16 mg/L based on measured concentrations.
Executive summary:

Chronic toxicity of TEA to Daphnia magna was tested according to German Federal Environmental Agency internal method, comparable to OECD TG 211.

The 21 d NOEC was 16 mg/Lbased on measured concentrations.