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EC number: 412-300-2 | CAS number: 139504-68-0 AMBER CORE
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
Description of key information
One key study is available on the bioaccumulation of Amber Core on Carp (Cyprinus carpio). BCFs of the test substance were estimated to have reached equilibrium after 8 weeks. To consider the worst case, the value used for chemical safety assessment was the highest BCF obtained: 173.
Key value for chemical safety assessment
- BCF (aquatic species):
- 173 dimensionless
Additional information
One key study is available on the bioaccumulation of Amber Core on Carp (Cyprinus carpio). This study was performed in a flow through system under GLP and according to the "Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body" stipulated in the "Testing Methods for New Chemical Substances" (July, 1974) and is based on OECD Guideline 305C (May, 1981).
An acute toxicity test was performed before the bioaccumulation test. This preliminary test result for 48 -hour LC50 (=9.95 mg/L) was used to decide to test concentrations of the test substance in the bioaccumulation study. Two test medium concentration levels were used: 0.050 mg/L and 0.0050 mg/L. The fish were acclimated and did not demonstrate any abnormalities during this period. The temperature of test water was maintained at 25 +/- 2°C with concentrations of dissolved oxygen in test tank comprised between 6.4 and 7.8 mg/L (corresponding to >60% saturation). 15 fish were used in Level 1 and 2 and 5 fish were used in Control (the control was set as a blank test). The duration of exposure was 8 weeks. 2 peaks on the mass fragmentogram were detected by GC-MS. The peaks were named peak A and B in the elution order respectively. The concentrations of test substance were analyzed by GC-MS.
BCFs of the test substance were estimated to have reached equilibrium after 8 weeks. The bioaccumulation potential of test substance in carp was an approximately similar value in both levels. No abnormality in behavior or appearance of test fish was noted. The average concentrations of the test substance in test water ranged from 66% to 75% at peak A and 74% to 88% at peak B respectively. Although these values were lower than a nominal concentration, the concentrations of the test substance were maintained at more than 90% in the preliminary test. To consider the worst case, the value used for chemical safety assessment was the highest BCF obtained: 173.
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