Barium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Due to technical problems the M4-medium used in the test was moderately different from the composition given in the test guideline. The deviations did not affect the test result.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium for about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (approximateiy 20 min at about 17700 G). The nominal concentration of the eluate was 100 mg/L.
- Eluate: yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna Straus
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation: 2-24 hours

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.2 - 3.2 mmol/L

Test temperature:

20.6 - 20.9 °C

pH:

8.0 - 8.2

Dissolved oxygen:

8.6 - 8.9 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 0, 100, 50, 25, 12.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water was used for culture and test purposes. This water was prepared on the basis of an ultrapure, demineralized water with a conductivity < 0.05 µS/cm. For the composition of this M4 medium see IS0 10706.

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (2-7 microeinstein/m*m*s) at 400 - 700 nm wave length

EFFECT PARAMETERS MEASURED: Mobility, visually determined after 0, 24, 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: no effects observed up to the limit of water solubility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The substance had no inhibitory effect in the range of its water solubility.

Results with reference substance (positive control):

The 24h-EC50 of the control substance potassium dichromate was 1,00 mg/L. (Date of the last control experiment: 12 Dec 2000, project-no: 97/0242/50/52

Validity criteria fulfilled:

yes

Conclusions:

With high probability acutely not toxic to aquatic invertebrates.

Description of key information

With high probability acutely not toxic to aquatic invertebrates.

Additional information

In a guideline study following OECD 202 the acute toxicity of the substance to Daphnia magna was tested (BASF AG, 2001). The exposure was conducted in a static system. Based on the mobility of the animals after 48 hours no effects were observed up to the limit of water solubility (at a nominal loading rate of 100 mg/L).