Barium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical verification

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1.00 g test substance were added to 10 L test water and the mixture was homogenized with an ultra-turrax stirrer. Since the test substance was sparingly soluble in water, the test concentration was prepared 1 day before the test organisms were introduced into the test vessel (study start) and stirred during this time, to ensure that saturated concentration was used.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: breed in testing facility
- Age at study initiation: about 7 months
- Length at study initiation: 3.6 (3.4 - 3.7) cm
- Weight at study initiation: 0.54 (0.43 - 0.63) g
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum

ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): 0 % mortality during the last 7 days prior to test initiation

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

- Total hardness: about 2.5 mM (about 250 mg/L CaCO3)
- Acid capacity: about 5.5 mM

Test temperature:

22 - 23 °C

pH:

8.1 - 8.2

Dissolved oxygen:

8.4 - 8.5 mg/L

Nominal and measured concentrations:

The following nominal concentration were tested: 0, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel type: open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume (water depth about 17 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: ca. 0.5 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

EFFECT PARAMETERS MEASURED: mortality and symptoms

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no effects observed up to the limit of water solubility.

Validity criteria fulfilled:

yes

Conclusions:

With high probability acutely not toxic to fish.

Description of key information

With high probability acutely not toxic to fish.

Additional information

One study on the acute toxicity of the substance to fish is available. In this study, zebrafish (Danio rerio) were exposed to the substance for 96 hours in a static test system (BASF, 2000). At test termination no effects were observed up to the limit of water solubility (at a nominal loading rate of 100 mg/L).