2-phenylpropan-2-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Screening test, with multiple effects reported as one result

Data source

Reference

Reference Type:

publication

Title:

Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes

Author:

Applegate, V.C., J.H. Howell, A.E.,Jr. Hall, and M.A. Smith

Year:

1957

Bibliographic source:

Spec.Sci.Rep.Fish.No.207, Fish Wildl.Serv., U.S.D.I., Washington, DC(): -

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

not detailed

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-phenylpropan-2-ol

EC Number:

210-539-5

EC Name:

2-phenylpropan-2-ol

Cas Number:

617-94-7

Molecular formula:

C9H12O

IUPAC Name:

2-phenylpropan-2-ol

Test material form:

liquid

Test organisms

Test organisms (species):

other: Lepomis macrochirus, Oncorhynchus mykiss and Petromyzon marinus

Details on test organisms:

Observations on the toxic effect of compounds were made on larvae of the sea lamprey (Petromyzon marinus), rainbow trout (Salmo gairdnerii) and bluegill sunfish (Lepomis macrochirus). Larval lamphreys varied from 3 to 5 inches in total length. Test fishes were of fingerling size, 4 inches or slightly less in length. Every effort was made to keep size variation at a minimum. Larval lamphreys were collected by means of an electrci shocker in the Ocqueoc River, Michigan and were held in running water in aquaria and small races under conditions which simulated their natural stream habitat. Test fishes were obtained from the stocks of local state and federal fish hatcheries and were held in large raceways. These specimens were maintained in the best possible physical condition until used in the laboratory.

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Water (consistently clear and of relatively uniform quality) used in all tests was drawn from a supply pumped directly from Hammond Bay of Lake Huron. The suction line intake of this pumping sytems was located 250 feet offshore at a depth of about 9 feet.

Total exposure duration:

24 h

Test conditions

Test temperature:

55 Fahrenheit

pH:

7.5 - 8.2

Dissolved oxygen:

8.6;13.7 parts per million

Details on test conditions:

Durng a typical year while tests were being conducted (November 1953 to December 1954) , pH varied from 7.5 to 8.2, dissolved oxygen from 8.6 to 13.7 ppm and free carbon dioxide from 5.0 to 9 ppm.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Remarks on result:

other: see Any Other Information

Any other information on results incl. tables

Sublethal observations / clinical signs:

Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.

Executive summary:

Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.