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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Screening test, with multiple effects reported as one result

Data source

Reference
Reference Type:
publication
Title:
Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes
Author:
Applegate, V.C., J.H. Howell, A.E.,Jr. Hall, and M.A. Smith
Year:
1957
Bibliographic source:
Spec.Sci.Rep.Fish.No.207, Fish Wildl.Serv., U.S.D.I., Washington, DC(): -

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
not detailed
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Test organisms

Test organisms (species):
other: Lepomis macrochirus, Oncorhynchus mykiss and Petromyzon marinus
Details on test organisms:
Observations on the toxic effect of compounds were made on larvae of the sea lamprey (Petromyzon marinus), rainbow trout (Salmo gairdnerii) and bluegill sunfish (Lepomis macrochirus). Larval lamphreys varied from 3 to 5 inches in total length. Test fishes were of fingerling size, 4 inches or slightly less in length. Every effort was made to keep size variation at a minimum. Larval lamphreys were collected by means of an electrci shocker in the Ocqueoc River, Michigan and were held in running water in aquaria and small races under conditions which simulated their natural stream habitat. Test fishes were obtained from the stocks of local state and federal fish hatcheries and were held in large raceways. These specimens were maintained in the best possible physical condition until used in the laboratory.

Study design

Test type:
static
Water media type:
freshwater
Remarks:
Water (consistently clear and of relatively uniform quality) used in all tests was drawn from a supply pumped directly from Hammond Bay of Lake Huron. The suction line intake of this pumping sytems was located 250 feet offshore at a depth of about 9 feet.
Total exposure duration:
24 h

Test conditions

Test temperature:
55 Fahrenheit
pH:
7.5 - 8.2
Dissolved oxygen:
8.6;13.7 parts per million
Details on test conditions:
Durng a typical year while tests were being conducted (November 1953 to December 1954) , pH varied from 7.5 to 8.2, dissolved oxygen from 8.6 to 13.7 ppm and free carbon dioxide from 5.0 to 9 ppm.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Remarks on result:
other: see Any Other Information

Any other information on results incl. tables

Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.
Executive summary:

Repeated experiments were performed in which larvae, trout, and blue gills were exposed to the maximum concentration of each solvent that could occur in any screening test. No adverse effect on any species was observed at any time. Observations of each test were made approximately six times, at various intervals, during the 24-hour test period. At each observation, the condition of every test specimen was determined and recorded. Chronological histories were thus obtained of any symptoms of illness and the occurrence of death. Any chemical killing the larval lampreys in eight hours or less at a concentration of 5 ppm. (regardless of the effects on other fishes) was tested further at levels of 1 and 0.1 ppm. Water temperature, test period, and procedures were identical with those described for the initial test at 5 ppm.