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EC number: 293-927-7
CAS number: 91648-65-6
Daphnia magna_OECD 202: EL50(48h): 41 mg/L WAF RA_1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol (No CAS, no EC)_Daphnia magna_EU Method C.2: available results are not suitable to calculate the EL50, so it is estimated by the geometric mean EL0/EL100 = 50mg/L WAF RA_1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol (No CAS, no EC)_Daphnia magna_OECD 202/ISO 6341 15: EL50(48h): 10 - 50 mg/L WAF, approx. 36 mg/L WAFRA_1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol (No CAS, no EC)_Daphnia magna_OECD 202: an exact calculation of the EL50 cannot be done; EL50(48h) < 100 mg/L WAF
The acute toxicity of
1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen
peroxide and tert-nonanethiol (CAS 91648-65-6) towards Daphnia magna was
investigated according to OECD 202 / EU Method C.2 under certificated
GLP compliance (Vryenhoef, 2012). Daphnia magna is a freshwater
representative of a wide variety of natural habitats and can therefore
be considered as an important non-target organism in freshwater
ecosystems. Based on the poor water solubility of the test substance (<
0.1 mg/L, Fox, 2012), a modification of the standard method for the
preparation of aqueous media was performed. Using the approach of Water
Accommodated Fractions (WAFs), based upon an approach by several
important regulatory authorities in the EU and elsewhere, aqueous media
are prepared by mixing the test substance with water for a prolonged
period. Pre-study work showed that a preparation period of 24 hours was
sufficient to ensure equilibration between the test substance and the
water phase. After a 1-hour settlement period, the test substance is
phase separated by siphon and the test organisms exposed to the aqueous
phase or WAF. Following a preliminary range-finding test, twenty
daphnids (4 replicates of 5 animals) were exposed to the WAFs over a
range of nominal loading rates of 10, 18, 32, 56 and 100 mg/L for 48 h
at a temperature of 21 - 22 °C under static test conditions. The number
of immobilized Daphnia and any adverse reactions to exposure were
recorded after 24 and 48 hours. The water temperature was recorded
daily, dissolved oxygen concentration and pH were recorded at the start
and termination of the experiment. Immobilization of daphnids was
observed after 24 h and 48 h. After an exposure period of 24 h 50 % of
daphnids were immobilized at 56 mg/L WAF. Therefore, the EL50(24h) is
reported as 56 mg/L. After 48 h, all daphnids were immobilized at 56
mg/L WAF. Based on statistical interpolation, the EL50(48h) is
determined as 41 mg/L with 95 % confidence limits of 39 - 43 mg/L WAF.
The LOELR(48h) was considered to be 56 mg/L WAF and the NOELR(48h) is 32
was also used test organisms in experiments with the read-across
reaction products with hydrogen peroxide and tert-dodecanethiol (No CAS,
no EC). Caspers (1999) exposed young
daphnids for a duration of 48 hours to the substance under static
conditions. The photoperiod was chosen as 16 h light and 8 h dark.
Considering the heterogenous composition of the test substance, Water
Accommodated Fractions (WAFs) were prepared. No analysis was included.
Physico-chemical parameters were measured after 24 and 48 hours. The EC0
and EC100 values were determined directly from the study, while the EC50
was calculated from the dose-response curve obtained. Based on this, the
test material can not be classified distinctly due to the studies
conduction as limit test and unavailability of measured test
concentrations. However, the experiment is reliable with restrictions
and satisfies the guideline requirements for an acute toxicity study
with freshwater invertebrates.
test principles were applied by Bouillon (2006). In this experiment,
daphnids were exposed to control and test chemical at
nominal concentration of 12.5, 48.6, 171 and 1486 mg/L WAF for 48h.
Immobilization was observed daily. The 48h-EL50 was determined to be
10-50 mg/L WAF, thus approx. 36mg/L WAF. The report available is only a
short summary of the study and the real concentrations of the test
substance are unknown since no analytical analysis was included. Hence,
the reliability of the test is limited. Thus the EL50 value can not be
study, the toxicity towards the aquatic invertebrates was investigated
in a static limit test conducted in accordance to OECD 202 (Weyandt and
Lebertz, 2005). The nominal test concentration was 100 mg/L, whereby the
solution was prepared as Water Accommodated Fraction (WAF), although not
explicit mentioned in the study report. Immobilization was observed
daily. Physico-chemical parameters were recorded at the beginning and
the end of the experiment. The percentage of immoblized daphnids was 75
% after 24 h and 100 % after 48 h, respectively. Therefore, the EC0(48h)
as well as the EC50(48h) is < 100 mg/L WAF, whereas the EC100(48h) is >
100 mg/L WAF. No analytical measurements were performed. Based on this
study, the test material can not be classified distinctly due to the
studies conduction as limit test and unavailability of measured test
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