Isooctyl mercaptoacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

no data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods with acceptable restrictions No data on test design. Limited data on environmental conditions. No data on tested concentrations.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1974

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Standard acute method with one single administration by gavage

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexyl mercaptoacetate

EC Number:

231-626-4

EC Name:

2-ethylhexyl mercaptoacetate

Cas Number:

7659-86-1

Molecular formula:

C10H20O2S

IUPAC Name:

2-ethylhexyl sulfanylacetate

Details on test material:

- Name of test material (as cited in study report): Thioglykolsäure-2-äthylhexylester
- Analytical purity: technical quality
- Impurities (identity and concentrations): no relevant impurity

Test animals

Species:

rat

Strain:

other: conventional random strain from laboratory

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: conventional random strain from "Staatlichen Zentralstelle für Versuchstierzucht und -versorgung, Berlin, Lichtenberg."
- Age at study initiation: no data
- Weight at study initiation: between 150 and 180 g
- Fasting period before study: 18 h before study
- Housing:groups of 10 rats
- Diet (e.g. ad libitum): pellets of standard food of K type
- Water (e.g. ad libitum): ad libitum
- Acclimation period: no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

peanut oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 1/10 (v/v)

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: litterature data

Doses:

No data

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: every week
- Necropsy of survivors performed: yes
- Other examinations performed: body weight increase, necropsy

Statistics:

Calculation of LD50: Lichtfield and Wilcoxon for the days of application, and Deichmann and Leblanc until the end of deaths due to test substance.

Results and discussion

Preliminary study:

No preliminary study

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

303 mg/kg bw

95% CL:

259 - 355

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

334 mg/kg bw

95% CL:

250 - 446

Mortality:

No details available

Clinical signs:

other: No data.

Gross pathology:

At necropsy there were no pathological section findings.

Other findings:

Additionnal single sublethal doses were tested: 80 mg/kg bw.
At this dose, after 20 hours, no significant damages of the liver or kidneys were observed (relative and absolute organ weight of liver and kidneys, Hexobarbital sleeping time, serum creatinine rate, activity of Serum LAP and Serum aldolase, hemoglobine rate, urine volume, albumine rate in urine, macrosopic observation . At necropsy there were no pathological section findings and body weights were not adversely affected.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: other: REGULATION (EC) No 1272/2008 of 16 December 2008

Conclusions:

The resulting LD50 was 303 mg/kg for male and 334 mg/kg for female. Therefore the test substance should be classified as harmful if swallowed.

Executive summary:

In an acute oral rat toxicity studywith EHTG in peanut oil, the LD50 was 303 mg/kg bw (males) and 334 mg/kg bw (females). There were 70 animals assigned to this study. There were no adverse effects seen in any of the rats during the macroscopic examination at necropsy and body weights were not adversely affected. A single sublethal doses of 80 mg EHTG/kg bw produced no significant damage of the liver or kidneys in either sex.