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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

NOEC/EC10: > 932 mg/L

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
932 mg/L

Additional information

Data on the substance itself are not available. Data are available on a structural analogue of the substance, a trimellitate ester with mixed C8 -C10 side chains. This was investigated using a method developed by the testing laboratory. Principle of the method: 100 mL BOD bottles closable by means of ground stoppers are to be charged with the culture solution, the suspension of bacteria (Pseudomonas putida Migula) and the test substance in stepped concentrations, closed so as to leave no air bubbles and incubated for 5 -6 hours at 25 ± 2 °C in a shaking incubator. Batches free of test substance serve as control. The difference between the oxygen content of the solutions in the individual containers at time zero and after the incubation time gives the bacterial oxygen consumption. Comparison of the amounts of oxygen consumed in the control and test batches gives information about the concentration-dependent influence of the test substance on the oxygen consumption. For the test 2.0 g of the test substance was weighed in 20 mL neutralised emulsifier (nonylphenol 10 EO, 5 PO) and emulsified in a shaking incubator at 25 °C for 19 h at 200 rpm. 1 mL of this test solution was given into the test vessel (100 mL) for the highest test concentration (1000 mg/L, corresponding to 980 mg a.i./L). For further test concentrations the test solution was adequately diluted with emulsifying solution (dilution 1:10 each). The tested substance did not affect the bacterial respiration in the tested concentration range (1, 10, 100 and 1000 mg/L; corresponding to 1, 9.8, 98 and 980 mg a.i./L),: EC10 > 0.98 g a.i./L, EC50 > 0.98 g a.i./L. This corresponds to an EC10> 932 mg/L for the registered substance when differences in molecular weight are taken into account.