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EC number: 201-877-4
CAS number: 89-04-3
The LC50 after 48 h of 1,2,4-Benzenetricarboxylic acid,
mixed decyl and octyl triesters was greater than 1 mg/L, tested with Daphnia
Measured Concentrations of test chemicals during a 48h exposure
Nominal Concentration Measured
concentration(mg/L) Percent of nominal
0.006 < 0.006 - - -
Disp.Cont. < 0.006 <
0.006 - - -
89.7 96.4 103.6 89.7
Mortality or immobility of Daphnia
magna exposed to the test chemicals
(mg/L) Cumulative number of dead or immobilized Daphnia
Mortality or Immobility)
hour 48 hour
Acute immobilisation in Daphnia has been investigated in a 48 hour test
according to OECD test methods. Dissolution/dispersion agents were used
to increase concentration of the substance in the test medium. The EC50
(48h) was determined to be >100 mg/L and the NOEC was 100 mg/L, the
highest concentration investigated.
The given concentration refers to the estimated content
Remark: In the DOC-determination it was not possible to
distinguish between carbon from the test substance and carbon from the
The effect of 1,2,4-Benzenetricarboxylic acid, mixed
decyl and octyl triesters on the immobilisation of Daphnia magna had
been studied in accordance with OECD Guideline for Testing of Chemicals
No. 202 part I and EEC Methods for Determination of Ecotoxicity, Annex
to Directive 92/69/EEC.
Daphnia magna was exposed to 1,2,4-Benzenetricarboxylic
acid, mixed decyl and octyl triesters for an exposure time of 48 hours.
As the test substance is poorly soluble in water, a 1 g/L mixture with
synthetic freshwater was prepared. This mixture was stirred for 18 hours
and filtrated afterwards. The filtrate was analyzed by means of DOC
analysis and showed an initial content of 7.4 mg/L DOC. The filtrate was
used as stock solution. The analytical determination of stock and test
solution showed that they were not stable within 48 hours (deviation >
20%), therefore the geometric mean of the measured values after 0 and 48
hours for the test solution (3.2 mg/L) were used for evaluation.
No lethality or immobilisation of Daphnia magna > 5% was
observed at the tested concentration after 48 hours exposure.
Short-term toxicity to Daphnia: EC50 - >100 mg/L; NOEC - 100 mg/L
Tests with a structural analogue of the registered substance, a
trimellitate ester with mixed C8 -C10 side chains, showed no toxicity
when tested at, or above, the limits of water solubility.
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