Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-877-4
CAS number: 89-04-3
Further testing for trioctyl
benzene-1,2,4-tricarboxylate is not considered necessary on the basis
that the substance is considered readily biodegradable.
In general, achieving the degree and rate
of biodegradation necessary to fulfil the readily biodegradable criteria
structure of the test substance is intrinsically degradable by
individual or consortia of competent microorganisms capable of degrading
the test substance are both present in sufficient number at the
beginning of the test and they are also viable and active under the
conditions of the test, and
test substance is delivered to and maintained in the test system in such
a way as to permit sufficient bioavailability to the microorganisms
present in the test inoculum.
Information on the structural elements of
trioctyl benzene-1,2,4-tricarboxylate and the compound as a whole would
suggest the substance to be intrinsically biodegradable, therefore
satisfying condition a). This is seen in the EPISUITE BIOWIN v4.10
results for trioctyl benzene-1,2,4-tricarboxylate and its lower and
higher TM4 and TM10 analogues, where rapid aerobic biodegradation is
predicted for all three structures on the basis that they all contain
linear C4 terminal chain [CCC-CH3] and ester [-C(=O)-O-C] fragments.
Increasing molecular weight has the effect of lowering probability
outcomes, but the effect is trivial, with all three structures predicted
to be readily biodegradable, thereby supporting the conclusion that
trioctyl benzene-1,2,4-tricarboxylate is readily biodegradable.
More importantly, experimental test data
are available for analogue substances for read-across to trioctyl
benzene-1,2,4-tricarboxylate, the outcomes of which are additionally
influenced by factors b) and c). Available data for tributyl
benzene-1,2,4-tricarboxylate (Polynt-IT: 001 Key | Experimental result)
show that the core benzene-1,2,4-tricarboxylate entity is readily
biodegradable, even under MITI I (OECD 301C) conditions, when dosed in
the test system at 100 mg/L. Straight-chain aliphatic alcohols are also
readily biodegraded and extending the substituent butyl groups linearly
by a further two or four -CH2 units to trihexyl
benzene-1,2,4-tricarboxylate (Vertellus-UK: S-01) and trioctyl
benzene-1,2,4-tricarboxylate would not be expected to introduce a
structural hindrance to biodegradation of the substituent alkyl chains.
However, it is noted that incremental
extension of the alkyl chains quickly leads to lower aqueous solubility,
thereby influencing bioavailability of the test substance to the
microbial inoculum as determined by water solubility. The most soluble,
short alkyl chain analogue tributyl benzene-1,2,4-tricarboxylate was
completely mineralised (88% in 28 d, based on ThOD) and classified as
readily biodegradable in the OECD 301C (MITI I) test, whereas trioctyl
benzene-1,2,4-tricarboxylate was not (3% in 28 d, based on ThOD) under
identical conditions. Both tests are considered reliable and the
collection and preparation of the MITI inoculum is understood to be
under centralised control, with distribution to Japanese test facilities
following completion of the 3-month adaptation period. Inter-laboratory
variation in the composition and biochemical activity of the inoculum
was therefore most likely not a decisive factor in the disparate test
outcomes. A much higher degree of biodegradation (65% in 28 d based on
oxygen consumption, concluding ready biodegradability but failing the
10-d window) was achieved for trioctyl benzene-1,2,4-tricarboxylate by
applying the van Ginkel modification of the OECD 301D Closed Bottle
Test, with a reduced concentration (2 mg/L as opposed to 100 mg/L in
OECD 301C), substitution of the standard, synthetic mineral salts medium
with a natural river water containing an indigenous microflora already
adapted to its environment, and the application of a poorly degradable
dispersing surfactant (Tween 80) to enhance test substance
Moreover, the proposed ester cleavage
mechanism that represents the key first step in the biodegradation of
TM8 entails the release of 1-octanol and trimellitic acid, and reliable
studies are available for octan-1-ol (Kao Chemical Europe S.L.-ES: 001
Key | Experimental result) and trimellitic acid (Polynt-IT: 001 Key |
Experimental result) which conclude that both substances are readily
In accordance with REACH Regulation
1907/2006/EC (Annex IX - 220.127.116.11 & 18.104.22.168 - column 2) simulation tests
of biodegradation in water and sediment do not need to be conducted as
the substance can be regarded as biodegradable via common microbial
pathways although the rate of degradation is likely to be limited due to
limited bioavailability as a result of low water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
På den här webbplatsen används kakor. Syftet är att optimera din upplevelse av den.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again