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EC number: 219-372-2
CAS number: 2425-85-6
The effect of the test item C.I. Pigment Red 3 on the survival, and
reproduction of Daphnia magnawas investigated in a semi-static
test over 21 days following the OECD Guidelines for Testing of
Chemicals, No. 211 (2008): “Daphnia magnaReproduction
Test”, the EU Commission Directive 92/69/, C.20: “Daphnia
magna Reproduction Test” and the Commission Regulation (EC) No
440/2008, C.20: “Daphnia magna Reproduction Test”.
The nominal concentrations tested were 0.45, 1.4, 4.5, 14, and 45 μg/L.
Additionally, a control and a solvent control were tested in parallel.
The preparation of the test media was based on the OECD Guidance
Document on Aquatic Toxicity Testing of Difficult Substances and
The concentration of the test item measured in freshly prepared test
medium of the nominal test concentrations of 45 μg/L was between 93 and
101 % of the nominal value. This shows the correct dosage of the test
item. At the end of the renewal periods, 50 to 80 % of nominal were
found in the samples with and without food.
For the nominal test concentration of 45 μg/L, the mean
measured test item concentration was calculated as thetime-weighted
meansover all measurements. Thereby, samples from the actual test
(with food) were taken into account.
The biological results are based on the mean measured
test item concentration of 35 μg/L. This measured
concentration is clearly above the water solubility of the test item as
provided by the sponsor.
The test item C.I. Pigment Red 3 had no toxic effects on survival and
reproduction of Daphnia magna after the exposure period of 21
days up to the highest test concentration of mean measured 35 μg/L
(nominal 45 μg/L). This test concentration is clearly above the water
solubility of the test item. The 21-day NOEC of the test item was
determined to be at least 35 μg/L. The 21-day LOEC was above the highest
test concentration of 35 μg/L.
Thus, the test item C.I. Pigment Red 3 had no toxic effects on Daphnia
magna at least up to its solubility limit in test water.
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