Registration Dossier
Registration Dossier
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EC number: 202-597-5 | CAS number: 97-63-2
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 006
Materials and methods
- Principles of method if other than guideline:
- Examination of the influence on cell viability using L-929 mouse fibroblasts. Methylmethacrylate (MMA), Ethyl methacrylate (EMA)and Lauryl methacrylate (LMA) and a new novel acrylic resin monomer were investigated. Medium containing each monomer was changed every 15 min as some monomers were volatile. After one hour of exposure, these mediums were replaced with a normal medium and cells were further incubated for 72 h. IC50 value for each monomer was determined, and chronological cell viability and cytomorphologic observation were evaluated.
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Ethyl methacrylate
- EC Number:
- 202-597-5
- EC Name:
- Ethyl methacrylate
- Cas Number:
- 97-63-2
- Molecular formula:
- C6H10O2
- IUPAC Name:
- ethyl methacrylate
- Details on test material:
- Purity not specified, but > 98% commercial grade assumed.
Constituent 1
Results and discussion
- Details on results:
- The cytotoxic effect of EMA on the cell viability of L-929 mouse fibroblasts was IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5)).
Applicant's summary and conclusion
- Conclusions:
- All monomers showed a dose-dependent manner in respect of the cell viability of L-929 mouse fibroblasts.
Methyl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Ethyl methacrylate: IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Lauryl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5)) - Executive summary:
All monomers showed in vitro a dose-dependent manner in respect of the cell viability of L-929 mouse fibroblasts.
Methyl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5)) Ethyl methacrylate: IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Lauryl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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