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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2006

Materials and methods

Principles of method if other than guideline:
Examination of the influence on cell viability using L-929 mouse fibroblasts. Methylmethacrylate (MMA), Ethyl methacrylate (EMA)and Lauryl methacrylate (LMA) and a new novel acrylic resin monomer were investigated. Medium containing each monomer was changed every 15 min as some monomers were volatile. After one hour of exposure, these mediums were replaced with a normal medium and cells were further incubated for 72 h. IC50 value for each monomer was determined, and chronological cell viability and cytomorphologic observation were evaluated.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Supplier: Sigma-Aldrich, Japan KK, Tokyo, Japan
- Purity: commercial grade assumed

Results and discussion

Details on results:
The cytotoxic effect of EMA on the cell viability of L-929 mouse fibroblasts was IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5)).

Applicant's summary and conclusion

Conclusions:
All monomers showed a dose-dependent manner in respect of the cell viability of L-929 mouse fibroblasts.
Methyl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Ethyl methacrylate: IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Lauryl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))
Executive summary:

All monomers showed in vitro a dose-dependent manner in respect of the cell viability of L-929 mouse fibroblasts.

Methyl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5)) Ethyl methacrylate: IC50: 12 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))

Lauryl methacrylate: IC50: 6.9 mmol/L (50 % depression of cell viability when compared with medium after 24 hours of incubation (n=5))