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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report Date:
1967

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
BASF-Test, see details in the section "Any other information on materials and methods incl. tables".
GLP compliance:
no
Remarks:
study was performed prior to GLP
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: Morpholine (1, 4-Tetrahydrooxazin)
- Physical state: liquid
- Analytical purity: 99.2 %
- Impurities: 0.2 to 0.3 % water, 0.1 % diethylene glycol, 0.4 % n-ethylmorpholine

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 151 g (mean)

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
not specified
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Please refer to the section "Any other information on materials and methods incl. tables".
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
1 h
Remarks on duration:
additional exposure times 3 h and 5.5 h
Concentrations:
1 h exposure: 35.1 mg/L air
3 h exposure: 28.8 mg/L air
5.5 h exposure: 21.14 mg/L air
No. of animals per sex per dose:
1 h exposure: 6
3 h and 5.5 h exposure: 3
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: several times on the day of exposure and daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
other: Inhalation Risk Test
Effect level:
other: Inhalation Risk Test
Exp. duration:
1 h
Remarks on result:
other: additional exposure times: 3 h and 5.5 h
Mortality:
1 h exposure: 0/12
3 h exposure: 2/6
5.5 h exposure: 6/6
Clinical signs:
other: Escape attempts, distinct irritation of mucous membrane, severe nose and eye discharge, dyspnoea, convulsions, widespread corrosion of nose and paws.
Body weight:
No evaluable data.
Gross pathology:
Animals that died: wet, ruffled fur, corrosive smell.
Sacrificed animals: no abnormalities observed.

Any other information on results incl. tables

The inhalation of a highly saturated vapor-air-mixture caused mortality after 3 h of exposure. There was indication that the test substance caused local irritation to exposed tissues including respiratory tract.

Applicant's summary and conclusion

Executive summary:

In an acute inhalation toxicity study according to an internal BASF method (BASF AG, 1967), male and female rats were exposed to Morpholine (99.4 %) vapour for a period of 1, 3 or 5.5 hours and observed for 7 days. All animals were subjected to necropsy. Exposure to Morpholine at vapour saturation concentrations resulted in 100 % lethality after 5.5 hours. Morpholine had irritating and corrosive properties.

This acute inhalation toxicity study is classified as acceptable. It satisfies the guideline requirement for an acute oral study according to OECD 403 in principle.