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Short-term toxicity to aquatic invertebrates

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Description of key information

Acutely harmful to aquatic invertebrates.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
45 mg/L

Additional information

To determine the toxicity of morpholine to aquatic invertebrates, a study following OECD 202 using Daphnia magna was performed. Based on nominal, analytically verified test concentrations a 48-h EC50 of 45 mg/L was determined (NITE, 1997). This value was selected as key data.

The key data are supported by the results of a study conducted according to DIN 38412, part 11 with D. magna as test species (Bringmann & Kuehn, 1977). However, the exposure duration was 24 instead of 48 hours. Based on nominal test concentrations (analytically not verified) a 24-h EC50 of 100 mg/L was determined.

Further supporting data are available from another study conducted according to DIN 38412, part 11 with D. magna as test species (Bringmann & Kuehn, 1982). The exposure duration was 24 instead of 48 hours. Based on nominal test concentrations (analytically not verified) a 24-h EC50 of 101 mg/L was found.

In both studies performed by Bringmann & Kuehn the stability of morpholine (CAS 110-91-8) in the test medium has not been analytically confirmed, but is considered to be stable due to the high water solubility (completely miscible), the moderate vapour pressure (VP = 9.8 hPa, at 20 °C) and the low Henry´s law constant (HLC, uncharged: 0.0115 Pa*m3/mol, at 25 °C) of the substance. "

In addition Calamari et al. (1980) conducted an AFNOR-immobilisation test with D. magna (comparable to DIN 38412, part 11). After an exposure period of 24 hours the EC50 was determined to be 119 mg/L (nominal, analytically verified).

In conclusion, morpholine is assessed to be acutely harmful to aquatic invertebrates.