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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1954
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Range-finding toxicity Data List V
Author:
Smyth HF, Carpenter CP, Weil CS & Pozzani UC
Year:
1954
Bibliographic source:
Arch. Ind. Hyg Occup. Med. 10 61–68

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
according to Draize et al. (1944)
GLP compliance:
no
Remarks:
prior to GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Morpholine
EC Number:
203-815-1
EC Name:
Morpholine
Cas Number:
110-91-8
Molecular formula:
C4H9NO
IUPAC Name:
morpholine
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.5 - 3.5 kg

Administration / exposure

Type of coverage:
occlusive
Vehicle:
not specified
Details on dermal exposure:
Penetration of rabbit skin was estimated by the one-day cuff method of Draize and associates. The fur was closely clipped over the entire trunk, and the close, retained beneath an impervious plastic film, contacted about 1/10 of the body surface.
Duration of exposure:
24 hours
Doses:
up to 20 mL/kg bw
No. of animals per sex per dose:
4 animals
Control animals:
not specified
Details on study design:
After 24 hours contact the film was removed. Mortality was considered complete after 14 additional days.
Statistics:
The most probable LD50 was estimated by the methods of Thompson (1947) and Weil (1952).

Results and discussion

Effect levelsopen allclose all
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 500 mg/kg bw
Remarks on result:
other: ≈ 0.5 mL/kg bw
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
>= 0.31 - <= 0.81 mL/kg bw
Remarks on result:
other: Limits of +/- 1.96 standard deviation using the method of Thompson
Mortality:
Death occurred within 14 days.
Clinical signs:
No data.
Body weight:
No data.
Gross pathology:
No data.

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria