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EC number: 240-367-6
CAS number: 16260-09-6
Results of non-GLP range-finding Test
Table 1: Shoot fresh weight of seedlings grown in test item treated soil
as determined on day 14 of the range-finding test; data given as
percentage of the control.
Test concentration [mg/kg soil]
Shoot fresh weight [% of control]
Results of main test:
The test with A. sativa was repeated, since the validity criteria
had not been met in first run. Only the results of the repeated test
with A. sativa are reported. The tests with the species A. cepa, P.
sativum and S. lycopersicum (only results of 2nd run are shown here)
were both valid considering the validity criteria given by the test
guideline. Consequently the results of both test runs were evaluated
separately and are reported.
Seedling Emergence and Survival
Emergence in the control was at least 75%. Survival of seedlings
in the controls was at least 90%. Hence, the requirements defined by the
test guideline regarding emergence and survival of seedlings were
fulfilled. Neither emergence nor survival of the seedlings was
statistically significantly affected in any of the species at test item
concentrations up to and including 1000 mg/kg soil dry weight which was
the highest tested concentration.
Seedling Shoot Fresh Weight
Shoot fresh weight of plants growing in test item treated soil was not
statistically significantly different from shoot weight of the plants
growing in untreated control soil for any of the six species at
concentrations up to and including 1000 mg/kg soil dry weight. In the
repeated test, however, shoot fresh weight of S. lycopersicum was
statistically significantly reduced by 20% and 27% at test item
concentrations of 316 and 1000 mg/kg soil dw, respectively.
Table 1: Seedling emergence (emergence after day 7 not considered)
Test item [mg/kg soil dw]
Number of seeds planted
A. cepa (2nd run)
P. sativum (2nd run)
S. lycopersicum (2nd run)
Number of emerged seeds per treatment on day 7
Relative number of emerged seeds [% of seeds sown]
Mean % emergence as % of mean emergence of the control
*Accidentally no seeds sowed in one of the pots of this treatment.
Table 2: Seedling survival (emergence after day 7 not considered)
A. cepa (2ndrun)
P. sativum (2ndrun)
S. lycopersicum (2ndrun)
Number of live seedlings at test end
Relative number of live seedlings at test end [% of emerged]
Mean % survival as % of mean survival of the control
EC10 (21 d): 78.5 mg/kg soil (Solanum lycopersicum, shoot fresh weight,
OECD 208, nominal)
One experimental study is available investigating the potential effects
of the test item (CAS 16260-09-6) on seedling emergence and growth (ECT,
2017) of plants. The studies were performed according to OECD guideline
208 under GLP conditions. For this study six species of non-target
terrestrial plants (2 monocots and 4 dicots) were planted in a natural
sandy loam soil (standard soil LUFA Sp 2.3) immediately after test item
application. Five test item concentrations were tested with each
species: 10, 31.6, 100, 316 and 1000 mg test item /kg soil dw. Plants
were left to grow under controlled conditions for a period of 14 to 21
days following seedling emergence of at least 50% in the control. Test
item concentrations were applied within artificial soil. The control
soil received sand which had not been treated with the test item. Soils
were supplied with water and a nutrient solution as needed by bottom
watering. The test was performed in growth chambers equipped with
artificial lighting (201 to 352 μE m-2 s-1, for 16 hours per day) and
air conditioning (mean air temperature ranging from 17.1 to 26.4 °C,
relative humidity ranging from 17.0% to 58.1%). The pots were randomly
placed at the beginning of the test and were re-arranged at minimum
weekly intervals. On day 7 and day 14 (or day 17 for A. cepa) after 50%
emergence of control, seedlings were evaluated visually. At the end of
the test, seedlings were counted, evaluated visually, and harvested to
determine shoot fresh weight. At concentrations up to and including 1000
mg/kg soil dry weight the test item had no statistically significant
adverse effect on either seedling emergence, seedling survival or shoot
fresh weight of any of the six test species. Shoot fresh weight of the
test species Solanum lycopersicum in the second test run was reduced at
concentrations of ≥ 100 mg/kg soil dry weight (P<0.05). Therefore the
lowest LOEC (lowest observed effect concentration) and NOEC (no observed
effect concentration) were determined to be 316 mg/kg soil dw and 100
mg/kg soil dw, respectively. Due to the lack of either adverse effects
or a concentration response relationship effective concentrations could
not be calculated and are assumed to be higher than 1000 mg/kg dw except
for shoot fresh weight of S. lycopersicum (second test run). For S.
lycopersicum the EC10 and EC25 were calculated to be 78.5 mg/kg soil dry
weight and 745 mg/kg soil dry weight, respectively, while the EC50 was
>1000 mg/kg soil dry weight under the conditions of this test.
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