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EC number: 240-367-6
CAS number: 16260-09-6
The test substance concentration was analysed for verification of test
concentrations at 0 and 72 hours by HPLC-MS-MS (Perkin Elmer, API 3000;
triple stage quadrupole mass spectrometer). The test concentration at 0
hours was below the limit of quantitation (LOQ= 0.014 mg/L). At 72 hours
a test concentration of 0.015 mg/L was measured.
No toxicity up to the limit of water solubility.
A study was performed to assess the
effect of the test item on the growth of the green alga Pseudokirchneriella
subcapitata.The method followed was designed to be compatible with
the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater
Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3
of Commission Regulation (EC) No 761/2009.
The test substance is highly insoluble
in water and pre-study solubility work conducted indicated that it was
not possible to obtain a testable solution of the test item using
traditional methods of preparation e.g. ultrasonication and high-shear
mixing. A pre-study media preparation trial indicated that the use of a
saturated solution method of preparation was most appropriate for this
Exposure of Pseudokirchneriella
subcapitata to the test item gave EC50
values of greater than 100% v/v
saturated solution. The No Observed Effect Concentration was 100% v/v
Analysis of the 100% v/v saturated
solution test preparation at 0 hours showed a measured test
concentration of less than the limit of quantitation (LOQ) of the
analytical method employed, which was determined to be 0.014 mg/L was
obtained. Analysis of the test preparation at 72 hours showed a measured
test concentration of 0.015 mg/L was obtained. This study showed that
there were no toxic effects at saturation.
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