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EC number: 240-367-6
CAS number: 16260-09-6
Terrestrial data of octadec-9-enylhexadecan-1-amide (oleyl
palmitamide, CAS 16260-09-6) are available for three trophic levels. All
studies were performed according to international guidelines and GLP.
One experimental study is available investigating the
toxicological effects of the test item (CAS 16260-09-6) to earthworms
(ECT, 2017). The study was performed according to OECD guideline 222.
Adult Eisenia andrei (4 x 10 animals per concentration, the fresh weight
was between 250 and 600 mg) were exposed for 28 days in an artificial
soil to the nominal concentrations of 62.5, 125, 250, 500 and 1000 mg
test item/kg artificial soil dry weight. The artificial soil comprised
5% sphagnum peat, 20% kaolin clay, 74–75% Quartz sand and 0.3 - 1%
calcium carbonate. No morphological and behavioral effects were
observed. Related to weight alterations and symptoms, the
no-observed-effect-concentration (NOEC) was ≥ 1000 mg test item/kg dry
weight soil, the lowest-observed-effect-concentration (LOEC) > 1000 mg
test item/kg dry weight soil for all parameters after 28 days for
mortality and biomass. No mortality was recorded resulting in a EC50 (28
d) of > 1000 mg test item/kg dry weight soil. For reproduction the no
observed effect concentration (NOEC) was observed at ≥ 1000 mg test
item/kg dry weight soil and the EC50 at > 1000 mg test item/kg dry
weight soil after 56 days.
No experimental data on the toxicity of
(Z)-N-octadec-9-enylhexadecan-1-amide (oleyl palmitamide, CAS
16260-09-6) to terrestrial arthropods are available. The substance is
characterised by a high log Koc (log Koc > 5) indicating a considerable
potential for adsorption to the soil particles but as the substance is
highly insoluble in water (< 0.01 mg/L), only low concentrations are
expected in the pore water. Therefore, tests with soil-dwelling
organisms like earthworm, which allow potential uptake via surface
contact, soil particle ingestion and porewater (ECHA, 2012), are most
relevant for the evaluation of soil toxicity. In addition, in the
absence of a clear indication of selective toxicity, an invertebrate
(earthworm or collembolan) test is preferred, as outlined in ECHA
guidance section R.184.108.40.206, page 122. Thus, it can be assumed that
earthworms would be highly exposed to toxicants in soil and hence are
most sensitive to the potential adverse effects of the substance.
One experimental study is available investigating the potential
effects of the test item (CAS 16260-09-6) on seedling emergence and
growth (ECT, 2017) of plants. The studies were performed according to
OECD guideline 208 under GLP conditions. For this study six species of
non-target terrestrial plants (2 monocots and 4 dicots) were planted in
a natural sandy loam soil (standard soil LUFA Sp 2.3) immediately after
test item application. Five test item concentrations were tested with
each species: 10, 31.6, 100, 316 and 1000 mg test item /kg soil dw.
Plants were left to grow under controlled conditions for a period of 14
to 21 days following seedling emergence of at least 50% in the control.
Test item concentrations were applied within artificial soil. The
control soil received sand which had not been treated with the test
item. Soils were supplied with water and a nutrient solution as needed
by bottom watering. The test was performed in growth chambers equipped
with artificial lighting (201 to 352 μE m-2 s-1, for 16 hours per day)
and air conditioning (mean air temperature ranging from 17.1 to 26.4 °C,
relative humidity ranging from 17.0% to 58.1%). The pots were randomly
placed at the beginning of the test and were re-arranged at minimum
weekly intervals. On day 7 and day 14 (or day 17 for A. cepa) after 50%
emergence of control, seedlings were evaluated visually. At the end of
the test, seedlings were counted, evaluated visually, and harvested to
determine shoot fresh weight. At concentrations up to and including 1000
mg/kg soil dry weight the test item had no statistically significant
adverse effect on either seedling emergence, seedling survival or shoot
fresh weight of any of the six test species. Shoot fresh weight of the
test species Solanum lycopersicum in the second test run was reduced at
concentrations of ≥ 100 mg/kg soil dry weight (P<0.05). Therefore the
lowest LOEC (lowest observed effect concentration) and NOEC (no observed
effect concentration) were determined to be 316 mg/kg soil dw and 100
mg/kg soil dw, respectively. Due to the lack of either adverse effects
or a concentration response relationship effective concentrations could
not be calculated and are assumed to be higher than 1000 mg/kg dw except
for shoot fresh weight of S. lycopersicum (second test run). For S.
lycopersicum the EC10 and EC25 were calculated to be 78.5 mg/kg soil dry
weight and 745 mg/kg soil dry weight, respectively, while the EC50 was
>1000 mg/kg soil dry weight under the conditions of this test.
One experimental study is available investigating the potential
effects of the test item (CAS 16260-09-6) on nitrogen transformation
(ECT, 2017) of terrestrial microorganisms.The study was performed
according to OECD guideline 216 under GLP conditions. Test item was
ground with fine quartz sand using a pestle and mortar prior to being
mixed together with the sand into the test soil (Lufa standard soil type
2.3). The soil test was amended with ground lucerne grass green meal
(5.0 g/kg soil). Test item was tested at five concentrations ranging
from 260 mg/kg soil dry weight (T1) to 1000 mg/kg soil dry weight (T5)
against an untreated control (C) with four replicates, each. Nitrate of
the soil was measured after test item application on day 0, and 28 days
after test item application. Due to the lack of a dose-response
relationship, effective concentrations could not be calculated. However,
based on the maximum effect of 16.7% observed at the highest tested
concentration, the EC50 and EC25 can be considered to be greater than
1000 mg/kg soil dry weight, which was the highest tested test item
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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