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Short-term and long-term studies investigating the aquatic toxicity of (Z)-N-Octadec-9-enylhexadecan-1-amide (CAS No. 16260-09-6) are available for three trophic levels (fish, aquatic invertebrates and algae) . No adverse effects on the test organisms were observed. Based on the available data (Z)-N-Octadec-9-enylhexadecan-1-amide is not considered to exhibit acute or chronic toxicity to aquatic organisms.

The results of the activated sludge respiration inhibition testing indicate no inhibition of the activity of sewage sludge organisms.