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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
genetic toxicity in vitro
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This study was classified as reliable without restriction because it is an acceptable and a well-documented study report.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Principles of method if other than guideline:
The method used for this study was a modified Ames test (ASTM E1687). Because the standard Ames test is considered to be inappropriate for testing the mutagenicity of water insoluble petroleum products, the Ames test was modified as described in Blackburn and co-workers (1986). Substances tested were considered positive if they showed a mutagenicity index (MI) greater than 1.0.
GLP compliance:
not specified
Type of assay:
other: Modified Ames assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
IUCLID4 Test substance: other TS: Kerosine, CAS No. 64742-81-0

Three samples were tested.
Sample CAS No. Treatment Source Crude
1 64742-81-0 hydrotreated North Sea
1 64742-81-0 hydrotreated Middle East
- Name of test material (as cited in study report): DMSO Extracts of kerosine
- Storage condition of test material: indoors under nitrogen blanket.
- Other: Temperature of storage space maintained above 0 degrees Celsius

Method

Species / strain
Species / strain / cell type:
other: S. typhimurium TA98
Metabolic activation:
not specified
Test concentrations with justification for top dose:
50 µl/mL

Results and discussion

Test results
Species / strain:
S. typhimurium TA 98
Metabolic activation:
not specified
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Remarks on result:
other: strain/cell type: S. typhimurium TA98
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The mutagenicity indices of the three samples were all zero, thus demonstrating an absence of mutagenic activity. Interestingly, none of the samples was found to contain any measurable 3-7 ring PACs.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

None of the kerosines tested were mutagenic.
Executive summary:

A standard Ames test was conducted on wet treated, straight-run kerosines and hydrotreated kerosines. S. typhimurium were exposed to kerosine and solvent DMSO at concentrations of 50 µL/mL. Because the standard Ames test is considered inappropriate for testing the mutagenicity of water insoluble petroleum products, the Ames test was modified according to Blackburn (1986) in order to be applied to straight-run kerosines. Substances tested were considered positive if they showed a mutagenicity index greater than 1.0. All three kerosines tested had MI values of 0.0.

This study received a Klimisch score of 1 and is classified as reliable without restrictions because it is an acceptable and a well-documented study report.