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EC number: 429-330-7
CAS number: -
The study procedures described in this
report were based on the IS0 Standard 6341, 1996-04-01. They were in
compliance with the EEC directive 92169, Part C.2. 'Acute toxicity for
Daphnia' and the OECD guideline No. 202: "Daphnia sp., Acute
Immobilisation Test', Adopted April 4, 1984. The water solubility of
PDTN was determined to be 1.66 g/l using the flask method. However, a
pretest showed that PDTN did not easily dissolve in the test medium. A
range-finding test was combined with a limit test exposing Daphnia for a
maximum of 48 hours to a range of nominal
concentrations from 0.1 to 100 mg/l. A stock solution o f 100 mg/l was
stirred for 69 hours before the start of the test. The highest test
concentration (100 mg/l) and the blankcontrol were tested in duplicate
with 10 daphnia per vessel. Samples for analysis were taken at the start
and the end of the test. Analysis of the samples taken during the limit
test showed that the measured concentration was in agreement with
nominal and remained stable during the test period. PDTN did not induce
acute immobilization of Daphnia magna at 100 mg/l after 48 hours of
exposure (NOEC). Hence, the EC50 was > 100 mg/l, the maximum
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