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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 207-079-2 | CAS number: 431-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published in peer reviewed literature, adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
- Objective of study:
- other: biotransformation
- Principles of method if other than guideline:
- Investigation of the biotransformation of the test substance in vivo in rats and in rat and human liver microsomes.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,1,1,2,3,3,3-heptafluoropropane
- EC Number:
- 207-079-2
- EC Name:
- 1,1,1,2,3,3,3-heptafluoropropane
- Cas Number:
- 431-89-0
- Molecular formula:
- C3HF7
- IUPAC Name:
- 1,1,1,2,3,3,3-heptafluoropropane
- Details on test material:
- HFA-227, 99.99% pure, based on flame-ionization detector response, supplied from Hoechst (Frankfurt, Germany)
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- three male Sprague-Dawley rats were used
Administration / exposure
- Route of administration:
- inhalation: vapour
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- Rats were individually exposed to 5000 ppm HFC 227ea in a closed, recirculating exposure chamber for 6 hr.
- Duration and frequency of treatment / exposure:
- Once for 6 hr
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5000 ppm
- No. of animals per sex per dose / concentration:
- 3 males
- Control animals:
- yes
- Positive control reference chemical:
- For the binding to human serum albumin experiment:
the acylating agent S-ethyltrifluoroacetate.
For the investigation regarding covalently binding to proteins in human liver microsomes:
a halothane metabolite - Details on study design:
- See section 'Details on dosing and sampling'.
- Details on dosing and sampling:
- After the exposure, the animals were transferred to an all-glass metabolic cage and urine was collected for 24 hr. On collection, the urine was quickly frozen at -78 C and stored at -20 C. To record NMR spectra, the urine was filtered tyrough Millipore HV filters (45 microm) and 400 microl) of urine were mixed to 50 microl of D2O. To quantitate microsomal metabolism of HFC 227ea to inorganic fluoride, 5 ml of supernatant from the microsomal incubation or 5 ml of urine were combined with an equal volume of total ionic strength-adjustment buffer and analyzed with a fluoride-selective electrode and a reference electrode. Samples were constantly stirred and vlaues for the samples were taken after a 10-minutes eqquilibration period. Fluoride contents were quantitated by comparing the values obtained with those of standard curves.
Furthermore, microsomal incubations of HFC 227ea were performed with rat and human liver microsomes.
In addition, the potential of hexafluoroacetone trihydrate, the only metabolite of HFC 227ea, to bind covalently with nucleophilic sites was investigated. - Statistics:
- Not specified
Results and discussion
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- No data
- Details on distribution in tissues:
- No data
- Details on excretion:
- No data
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- 19F-NMR identified hexafluoroacetone trihydrate in the urine of exposed rats. No significant increase of inorganic fluoride was observed in urinary excretion compared to untreated rats (0-48 hours after the end of the exposure). Because of the very low concentrations of hexafluoroacetone trihydrate present in the collected urine samples, the amount of this metabolite could not be quantified.
19F-NMR analysis of the supernatants of rat and human liver microsomes incubated with HFA-227ea showed consistent formation of fluoride only in liver microsomes from pyridine-pretreated rats and in two of the eight human liver samples. The rates of fluoride formation were very low and could not be quantified exactly (0.8 +/- 0.4 nmol/mg/20 min). The presence of traces of hexafluoroacetone trihydrate was indicated for the supernatants of incubations of HFA-227ea with liver microsomes from pyridine-retreated animals and for the two human liver samples catalyzing the formation of fluoride.
Any other information on results incl. tables
Because some aldehydes may covalently bind to proteins and the formation of fluorinated protein adducts has been implicated in immune-mediated hepatitis induced by halothane, the binding of hexafluoroacetone trihydrate to proteins was also investigated. Hexafluoroacetone trihydrate also gave only a very small resonance in fluorine NMR experiments when binding to human serum albumin was studied in comparison with the acylating agent S-ethyltrifluoroacetate. Moreover, no fluorine-containing products were formed by the reaction of hexafluoroacetone trihydrate with Nα-acetyl-L-Iysine, and hexafluoroacetone trihydrate was not metabolized to fluorine-containing metabolites or inorganic fluoride in rats. Comparative studies in human liver microsomes demonstrated that a halothane metabolite may covalently bind to proteins; in contrast, metabolism and covalent binding of HFA 227ea could not be demonstrated.
The data indicate that HFA-227 is biotransformed at very low rates to hexafluoroacetone trihydrate but irreversible binding of hexafluoroacetone trihydrate cannot be demonstrated, even with the application of very sensitive methods, and is considered unlikely, based on the combination of the results obtained.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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