2-(2-oxoimidazolidin-1-yl)ethyl methacrylate

Short-term toxicity to fish

Description of key information

The 96 hour LC50, value for ureido methacrylate with rainbow trout was >962 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

962 mg/L

Additional information

Key study:

A study was performed to assess the acute toxicity of ureido methacrylate to rainbow trout (Oncorhynchus mykiss) under semi-static, daily renewal conditions. This study was conducted in accordance with the test guidelines EC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC Part C, Method 1 "Acute toxicity for Fish" and OECD Guideline for Testing of Chemicals No. 203 "Fish, Acute Toxicity Test". Groups of seven juvenile fish were exposed to concentrations, nominally 46, 100, 220, 460 and 1000 mg/L of ureido methacrylate dissolved in water. Actual mean measured test concentrations were 42, 93, 201, 420, 962 mg/L. Measured concentrations ranged from 91 - 112% of nominal at 0 hours, 90 - 93% of nominal at 24 hours, 90 - 94% of nominal at 72 hours and 89 - 93% of nominal at 96 hours. Observations were made on the numbers of dead fish and the incidence of sublethal effects after 15 minutes and 2, 4, 24, 48, 72 and 96 hours exposure.

Sublethal reactions to exposure (other than death) were hyperventilation, coughing, erratic swimming, increased pigmentation, and resting on base of tank. These responses were observed only among fish exposed in the highest (962 mgll) treatment level. The 96 hour LC5, value for ureido methacrylate with rainbow trout was >962 mg/L. The 'no-observed effect concentration' for ureido methacrylate with rainbow trout was 420 mg/L.

Supporting study:

The acute lethal toxicity of ureido methacrylate (50 %) to Poecilia reticulata (Guppy) was investigated under semi-static exposure conditions over a period of 96 h. The only applied nominal test concentration was 100 mg/L active ingredient. Since the concentration of the active ingredient in the test material was 50 %, 200 mg/L test material were applied. The test medium was replaced every 24 h.

HPLC analyses of the test substance concentration were conducted at the beginning and at the end of every 24 h cycle. The analyses revealed that the test substance concentration was satisfactorily maintained (>80 %) over the whole incubation time of 96 h.

At the nominal concentration of 100 mg/L active ingredient none of a total of seven fish died after 96 h of exposure. No mortality was also observed in the blank control.

Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed. The no-observed-effect concentration (NOEC) was >100 mg/L active ingredient. 100 % mortality (LC100) could not be detected. Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is greater than the applied nominal test concentration of 100 mg/L active ingredient (>200 mg/L test material).

The acute lethal toxicity (OECD 203) of Hydroxyethylimidazolidone Methycrylate to Danio rerio was under static exposure over 96 hours. The LC50 was greater tahn 100 mg/l. (Arkema, 2003)