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EC number: 937-260-8
CAS number: -
Based on the analogue approach justification, the following results on the acute toxicity to fish obtained on Vinasses, residue of fermentation, can be applied to the Vinasses, residue of fermentation, depotassified :LC50(96h) > 100 mg/L (nominal); > 58 mg/L (measured)
study is available on Vinasses, residue of fermentation.The short-term
toxicity was tested according to the OECD Guideline 203 “Fish, Acute
Toxicity Test” (Bouwman, 2010).Cyprinus carpiowas used for
testing in a static limit test (loading 100 mg/L, nominal). After a test
duration of 96 h, no mortality or other abnormal responses were
observed. Based on the TOC content of Vinasses (i.e. 25.96%) the
concentration of Vinasses in the aquatic samples was calculated and
corrected for the control. Thus, the test resulted in an LC50 (96 h) of
> 58 mg/L based on the average exposure concentration.
test, dissolved oxygen concentration had dropped to 3.1 mg/L in the
limit concentration (after 2 days of exposure). Therefore, aeration was
introduced immediately. No fish mortality or any other clinical effects
were observed. Furthermore, after two days of exposure the limit
concentration was observed to be hazy. The following days undissolved
test substance was seen in the test vessel. The study author concluded
that the applied concentration was above the limit of water solubility
of Vinasses, residue of fermentation. There is no test on water
solubility of this test substance available. Whereas, for the similar
UVCB substance, Vinasses, residue of fermentation containing biomass ofCorynebacterium
glutamicum, it was shown that a large fraction of components with
relatively high water solubility is existent (Oudhoff 2010, OECD 105).
However, the test substance was not completely soluble in water since in
all samples undissolved test substance particles were observed.
Nevertheless, large fractions exhibited a very high water solubility
(38g/L at a loading rate if 100g/L, pH 3.5-3.6). Therefore, it can be
concluded, that the observed hazyness and partly precipitations are
rather a result of the variable composition of this test substance, than
of a limited water solubility. It is likely that both bacterial
activities together with the variable composition of the substance
resulted in the observed oxygen depletion and precipitation.
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