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EC number: 825-814-0
CAS number: 222721-94-0
In the acute immobilisation test with
Daphnia magna (STRAUS), the effects of a saturated solution with a
nominal loading of 100 mg/L of the test item 1,2,3 -Propanetricarboxylic
acid, 2 -Hydroxy-, tris(C12 -C13 -branched-alkyl)ester were determined
according to OECD 202 (2004) from 2015-06-01 to 2015-06-05, with the
definitive exposure phase from 2015-06-03 to 2015-06-05. The limit test
was conducted under static conditions over a period of 48 hours. The
test item is a clear and colourless liquid with a negligible solubility
in water. Therefore, a saturated solution with a nominal loading of 100
mg/L of the test item was prepared with ISO test water (acc. to OECD
202, Annex 3) by stirring with approximately 1100 rpm for 48 hours at
room temperature followed by filtration with a membrane filter (0.2 µm,
RC, MACHEREY-NAGEL) to achieve the saturated solution. The saturated
solution was visually clear throughout the exposure phase of 48 hours.
Twenty daphnids each were exposed to the limit concentration and the
control. The concentrations of 1,2,3 -Propanetricarboxylic acid,
2-Hydroxy-, tris(C12-C13-branched-alkyl)ester were analytically verified
via LC-MS/MS at the start of the exposure (0 hours) and at the end of
the exposure (48 hours) in the saturated solution and the control. With
regard to the measured concentrations in samples of the non GLP
preliminary range finding test with measured concentrations of 60 µg/L
and sensitivity of the analytical method, the limit of quantification
(LOQ) was set to 40 µg test item /L. However, the measured
concentrations of the test item at the start (0 hours) and at the end of
the exposure (48 hours) were below LOQ in the definitive study. This can
be attributed to the fact, that the water solubility of the test item is
very low (≤ 0.739 ng/L), lower than the LOQ. Since no effects on daphnia
magna were observed, the analytical results were acceptable. The effect
levels given are based on the nominal loading of the test item 1,2,3
-Propanetricarboxylic acid, 2 -Hydroxy-,
tris(C12-C13-branched-alkyl)ester. The water quality parameters (i.e.
pH-value and dissolved oxygen concentration), measured at the start (0
hours) and at the end of the exposure (48 hours), were within the
acceptable limits. The validity criteria of the test guideline were
fulfilled. At the saturated solution with a nominal loading of 100 mg/L
of the test item 1,2,3-Propanetricarboxylic acid, 2-Hydroxy-,
tris(C12-C13-branched-alkyl)ester, no effects were observed on Daphnia
magna. Thus, the test item is not toxic in the range of the water
In acute immobilisation tests with Daphnia
magna (STRAUS), the effects of a saturated solution of the similar
substance 1,2,3 -Propanetricarboxylic acid, 2 -hydroxy-, tris(C12 -13
-branched-alkyl) ester and the source substance Tri-(hexyl, octyl,
decyl) citrate resulted in no toxicity up to the range of water
solubility. The same is expected for 1,2,3-Propanetricarboxylic acid,
2-hydroxy-, tri-C14-15-alkyl esters (Please see Analogue Approach).
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