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EC number: 617-081-9
CAS number: 80473-92-3
For tables, please see attached file "Acute aquatic daphnia tables and
The acute toxicity of Thio-Acid to Daphnia magna was determined at
approximately 20C in a 48-hour static range-finding limit test.
The study was carried out in accordance with the requirements of Annex V
to Commission Directive 92/69/EEC: C.2. Acute toxicity for Daphnia and
the OECD Guideline for Testing of Chemicals No 202, Part 1, Daphnia sp.,
Acute Immobilisation Test.
The study was conducted as a range-finding limit test,whereby four
replicate vessels were prepared for the control and 100 mg/l treatments
with two replicate vessels at the remaining concentrations. Each vessel
contained five juvenile Daphnia magna. The 100 mg/L test media were
prepared by direct addition of 0.1001 g of Thio-Acid to 1 litre of ASTM
media. To aid the dispersion of the test article, the flask contents
were treated with ultrasound for 30 minutes. Following that, the flask
contents were placed onto a magnetic stirrer and stirred in the dark for
1 hour. The remaining exposure concentrations were prepared by serial
dilution of the 100 mg/L test medium.
No concentration related immobility of Daphnia magna was observed in the
control and all exposure concentrations during the range-finding limit
test. As there was no immobility observed during the range-finding limit
test, the 48-hour EC50 value and the corresponding no observed effect
concentration (NOEC) for Daphnia magna exposed to Thio-Acid could not be
determined. Therefore, the 48-hour EC50 value is considered to be
>100mg/L, the highest nominal exposure concentration tested in the
range-finding limit test. In error, observations of the Daphnia magna
were omitted at 24 hours. As there was no significant concentration
related immobility observed during the test, this omission does not
affect the integrity of the test.
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