(diethylamino)acetonitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

prior to GLP and OECD TG

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF test (internal method).

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

(diethylamino)acetonitrile

EC Number:

221-130-6

EC Name:

(diethylamino)acetonitrile

Cas Number:

3010-02-4

Molecular formula:

C6H12N2

IUPAC Name:

2-(diethylamino)acetonitrile

Details on test material:

Name of the test substance used in the study report: Produkt 73/127roh (= Diaethylaminoacetonitril roh)

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

- Weight at study initiation: 3.11 and 2.92 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

0.05 mL in the conjunctivae sac of the eye

Duration of treatment / exposure:

Single dose

Observation period (in vivo):

8 d

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

other: 24 h

Score:

> 1 - < 2

Max. score:

3

Reversibility:

not fully reversible within: 8 days

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

other: 24 h

Score:

0

Max. score:

3

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 24 h

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 8 days

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

other: 24 h

Score:

2

Max. score:

3

Reversibility:

fully reversible within: 8 days

Results 5

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

other: 24 h

Score:

1

Max. score:

3

Reversibility:

fully reversible within: 8 days

Results 6

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

other: 24 h

Score:

0

Max. score:

3

Irritant / corrosive response data:

One hour after dosing a slight cornea opacity and redness and slight to marked chemosis was observed. 24 hours after dosing slight chemosis and redness and slight to marked corneal opacity was observed in animal 1. In animal 2, marked redness was observed. All effects were reversible after 8 days except corneal opacity of rabbit 1, which was considerd to be slight. In addition, a greasy secretion from the eye was noted 1 hour after dosing in both animals and after 24 hours in animal 2.