(diethylamino)acetonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

prior to GLP and OECD TG

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

prior to GLP and OECD TG

Principles of method if other than guideline:

According to BASF-internal standard:
Several groups of young adult laboratory rats (5-10 per sex and dose) were treated simultaneously by gavage with preparations of the test substance in a suitable vehicle. The concentrations of these preparations were usually adjusted to achieve comparable volumes (e.g. 10 ml) per kg body weight. Group-wise documentation of clinical signs was performed over the 7- to 14- day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form. On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(diethylamino)acetonitrile

EC Number:

221-130-6

EC Name:

(diethylamino)acetonitrile

Cas Number:

3010-02-4

Molecular formula:

C6H12N2

IUPAC Name:

2-(diethylamino)acetonitrile

Details on test material:

Name of the test substance used in the study report: Product 73/127 dest. (= Diaethylaminoacetonitril dest.)

Test animals

Species:

rat

Strain:

other: Gassner rats

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

The test substance was administered in concentrations of 1 and 2%; aqueous emulsion with CMC.

Doses:

100, 160, 200, 400 µL/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 138.4 mg/kg bw

Based on:

test mat.

Remarks on result:

other: original values: LD50 is 160 µL/kg

Mortality:

100 µL/kg: no deaths after 7 days;
160 µL/kg: 5/10 deaths within 24 hours
200 µL/kg: 9/10 deaths within 24 hours
400 µL/kg: 10/10, 9/10 animals died within 15 minutes and after 2.5 hours the last animal died.

Clinical signs:

100 µL/kg: no symptoms
160 µL/kg: Accelerated breathing and after 15 minutes dyspnea and atony. The next day the surviving animals were symptom free.
200 µL/kg: Accelerated breathing, convulsions, staggering, abdominal cramps and dyspnea. After ca. 15 minutes atony.
400 µL/kg: Immediately after dosing: accelerated breathing, convulsions, staggering, abdominal cramps and dyspnea

Gross pathology:

The pathological finding in animals that died within 7 days after dosing were: acute heart dilatation and congestive hyperemia
There were no pathological findings in the surviving animals.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria