(diethylamino)acetonitrile

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Principles of method if other than guideline:

According to BASF-internal standard:
Several groups of young adult laboratory rats (5-10 per sex and dose) were treated simultaneously by injection of the test substance into the peritoneal cavity in a suitable vehicle. The concentrations of these preparations were usually adjusted to achieve comparable volumes (e.g. 10 ml) per kg body weight. Group-wise documentation of clinical signs was performed over the 7- to 14- day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form. On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(diethylamino)acetonitrile

EC Number:

221-130-6

EC Name:

(diethylamino)acetonitrile

Cas Number:

3010-02-4

Molecular formula:

C6H12N2

IUPAC Name:

2-(diethylamino)acetonitrile

Details on test material:

Name of the test substance used in the study report: Produkt 73/127 dest. (= Diaethylaminoacetonitril dest.)

Test animals

Species:

mouse

Strain:

other: Kisslegg mice

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

Test concentrations used were 0.1, 0.5, 2 or 16% (v/v); aqueous emulsion with CMC.

Doses:

12.5, 25, 32, 40, 50, 200, 1600 µL/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 26 mg/kg bw

Based on:

test mat.

Remarks on result:

other: original value: ca. 30 µL/kg

Mortality:

12.5 and 25 µL/kg: no deaths after 7 days
32 µL/kg: 6/10 deaths within 1 hour
40, 50, 200 and 1600 µL/kg: 10/10 within 1 hour

Clinical signs:

1600 - 50 µL/kg: Immediately after injection: increased breathing rate, dyspnea, staggering, convulsions, abdominal cramps.
40-25 µL/kg: Immediately after injection or after ca. 5 minutes: increased breathing rate, dyspnea, staggering, convulsions, abdominal cramps. Surviving animals were free of symptoms the next day.
12.5 µL/kg: increased breathing rate, crouched posture.

Gross pathology:

12.5- 50 µL/kg: no intra abdominal substance residues or adhesions
200 - 1600 µL/kg: congestive hyperemia